ArticleAnalytical biochemistry2004
Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces.
Article in Analytical biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 111 citations in OpenAlex.
- Silver Nanoparticles Improve Fluorophore Photostability: Application to a Hypericin Study.International journal of molecular sciences · 2024Article
- Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point-of-Care: A Review of Recent Technologies.Advanced materials (Deerfield Beach, Fla.) · 2023Review
- Cellulose Acetate-Based Plasmonic Crystals for Surface-Enhanced Raman and Fluorescence Spectroscopy.ACS materials Au · 2022Article
- A straightforward and sensitive "ON-OFF" fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence.RSC advances · 2021Article
- Preparation of plasmonic platforms of silver wires on gold mirrors and their application to surface enhanced fluorescence.ACS applied materials & interfaces · 2014Article
- Silver nanoparticle-enhanced fluorescence in microtransponder-based immuno- and DNA hybridization assays.Analytical and bioanalytical chemistry · 2010Article
- Plasmonic platforms of self-assembled silver nanostructures in application to fluorescence.Journal of nanophotonics · 2010Article
- A microemulsion preparation of nanoparticles of europium in silica with luminescence enhancement using silver.Nanotechnology · 2009Article
- Near-infrared squaraine dyes for fluorescence enhanced surface assay.Dyes and pigments : an international journal · 2009Article
- Metal Enhanced Fluorescence on Silicon Wafer Substrates.Chemical physics letters · 2008Article
- Single molecule studies of multiple-fluorophore labeled antibodies. Effect of homo-FRET on the number of photons available before photobleaching.Current pharmaceutical biotechnology · 2008Article
- A novel method for monitoring monoclonal antibody production during cell culture.Biotechnology and bioengineering · 2008Article
- Enhanced fluorescent immunoassays on silver fractal-like structures.Analytical chemistry · 2008Article
- Fluorescence Quenching/Enhancement Surface Assays: Signal Manipulation Using Silver-coated Gold Nanoparticles.Chemical physics letters · 2008Article
- Fluorescence spectral properties of rhodamine 6G at the silica/water interface.Journal of fluorescence · 2008Article
- Metal-Enhanced Fluorescence of Phycobiliproteins from Heterogeneous Plasmonic Nanostructures.The journal of physical chemistry. C, Nanomaterials and interfaces · 2007Article
- Metal particle-enhanced fluorescent immunoassays on metal mirrors.Analytical biochemistry · 2007Article
- Metal-enhanced fluorescence of an organic fluorophore using gold particles.Optics express · 2007Article
- One- and two-photon induced fluorescence of Pacific Blue-labeled human serum albumin deposited on different core size silver colloids.Biopolymers · 2006Article
- Myoglobin immunoassay based on metal particle-enhanced fluorescence.Journal of immunological methods · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We present a generic immunoassay platform that uses enhanced total internal reflection fluorescence in the proximity of silver island films (SIFs), a surface coating consisting of metal (silver) particles. This platform is used with a model immunoassay where a protein antigen, rabbit immunoglobulin G, was immobilized on the SIF-coated glass surface. The signal from a fluorescent dye-labeled anti-rabbit antibody binding to the surface antigen was detected; different color dyes have been tested. Close placement of the fluorophore to surface-bound silver nanostructures results in dramatic signal enhancement (up to 40-fold) on the SIFs as compared with the glass slides. Use of the total internal reflection mode of excitation has significant advantages (over classic front-face excitation) for practical assay development. The limited evanescent wave excitation volume makes it possible to minimize the background signal and use the immunoassay with no need for any washing steps.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.