ArticleAnnals of surgery2004
Tissue-engineered small intestine improves recovery after massive small bowel resection.
Article in Annals of surgery, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.
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Who cites it
83 citing papers in PubMed, 1 synthesis or guideline pooled it, 219 citations in OpenAlex.
- Current practice and future perspectives in the treatment of short bowel syndrome in children--a systematic review.Langenbeck's archives of surgery · 2012Pooled it
- Functional Imaging for Regenerative Medicine: Tracking Vascularization of Intestinal Scaffolds.Tissue engineering. Part A · 2026Article
- Organoid Technology as a Promising Frontier in Organ Transplantation and Regenerative Medicine.International journal of stem cells · 2026Review
- Synthesis and Characterization of Biomimetic Thermoplastic Polyurethanes and Nanocomposites with l-Lysine Diisocyanate.Biomacromolecules · 2026Article
- Advancing precision triage in strangulated small bowel obstruction: from static scores to dynamic multiparametric models.Frontiers in surgery · 2026Review
- Development of a novel tubular scaffold for tissue-engineered small intestine.Journal of pediatric surgery · 2026Article
- Engineering hope: tissue-engineered intestine as a breakthrough for pediatric short bowel syndrome.Annals of medicine and surgery (2012) · 2025Article
- Bioengineering of Intestinal Grafts.Gastroenterology clinics of North America · 2024Review
- Tissue engineering and regenerative medicine approaches in colorectal surgery.Annals of coloproctology · 2024Review
- Stem cells as a therapeutic avenue for active and long-term complications of Necrotizing Enterocolitis.Seminars in pediatric surgery · 2023Article
- Current status and clinical applications of tissue engineering of the gastrointestinal tract: a systematized narrative review.Frontiers in gastroenterology (Lausanne, Switzerland) · 2023Review
- Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022Review
- Evidence of glucose absorption in a neoformed intestine.Updates in surgery · 2022Article
- Building gut from scratch - progress and update of intestinal tissue engineering.Nature reviews. Gastroenterology & hepatology · 2022Review
- Tissue engineering of the gastrointestinal tract: the historic path to translation.Journal of biological engineering · 2022Review
- Innovations in Immunosuppression for Intestinal Transplantation.Frontiers in nutrition · 2022Review
- In Vivo Transplantation of Human Intestinal Organoids Enhances Select Tight Junction Gene Expression.The Journal of surgical research · 2021Article
- R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and SurvivalFrontiers in bioengineering and biotechnology · 2020Article
- Building Scaffolds for Tubular Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Development of Intestinal Scaffolds that Mimic Native Mammalian Intestinal Tissue.Tissue engineering. Part A · 2019Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveRescue with tissue-engineered small intestine (TESI) after massive small bowel resection (MSBR). SUMMARY BACKGROUND DATA: Short bowel syndrome is a morbid product of massive small bowel resection. We report the first replacement of a vital organ by tissue engineering with TESI after MSBR.
methodsTen male Lewis rats underwent TESI implantation with green fluorescent protein (GFP)-marked cells (TESI+, n = 5) or sham laparotomy (TESI-, n = 5) followed by MSBR. Side-to-side anastomosis of TESI to proximal small intestine was performed or omitted. TESIO animals underwent implantation of engineered intestine with no further surgery. Weights were measured QOD until day 40. Transit times were measured. DNA assay was performed with computer morphometry. Northern blots of RNA were probed for intestinal alkaline phosphatase (IAP) and villin. Hematoxylin and eosin, S100, and smooth muscle actin immunohistochemistry were performed. Blood was collected at sacrifice.
resultsAll 10 rats initially lost then regained weight. The initial rate of weight loss was higher in TESI+ versus TESI-, but the nadir was reached a week earlier with more rapid weight gain subsequently to 98% preoperative weight on day 40 in animals with engineered intestine versus 76% (P < 0.03). Serum B12 was higher at 439 pg/mL versus 195.4 pg/mL. IAP mRNA appeared greater in TESI+ than TESIO, with constant villin levels. Histology revealed appropriate architecture including nerve. GFP labeling persisted.
conclusionsAnastomosis of TESI significantly improved postoperative weight and B12 absorption after MSBR. IAP, a marker of differentiation in intestinal epithelium, is present in TESI, and GFP labeling was accomplished.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.