ArticleThe Journal of cell biology2004
Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Integrated control of formin-mediated actin assembly by a stationary inhibitor and a mobile activator.The Journal of cell biology · 2018Article
- Cytoskeletal impairment during isoamyl alcohol-induced cell elongation in budding yeast.Scientific reports · 2016Article
- A comprehensive model to predict mitotic division in budding yeasts.Molecular biology of the cell · 2015Article
- Spatial control of microtubule length and lifetime by opposing stabilizing and destabilizing functions of Kinesin-8.Current biology : CB · 2014Article
- Astral microtubule asymmetry provides directional cues for spindle positioning in budding yeast.Experimental cell research · 2012Review
- Molecular mechanisms of organelle inheritance: lessons from peroxisomes in yeast.Nature reviews. Molecular cell biology · 2010Review
- Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.Molecular biology of the cell · 2010Article
- Coordinating mitosis with cell polarity: Molecular motors at the cell cortex.Seminars in cell & developmental biology · 2010Review
- Spindle orientation during asymmetric cell division.Nature cell biology · 2009Review
- Article
- Role of Septin cytoskeleton in spine morphogenesis and dendrite development in neurons.Current biology : CB · 2007Article
- A novel pathway that coordinates mitotic exit with spindle position.Molecular biology of the cell · 2007Article
- Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.Eukaryotic cell · 2007Article
- The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.Molecular biology of the cell · 2007Article
- Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast.Molecular biology of the cell · 2006Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Asymmetric spindle positioning.Current opinion in cell biology · 2006Review
- Midbodies and phragmoplasts: analogous structures involved in cytokinesis.Trends in cell biology · 2005Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In Saccharomyces cerevisiae, spindle orientation is controlled by a temporal and spatial program of microtubule (MT)-cortex interactions. This program requires Bud6p/Aip3p to direct the old pole to the bud and confine the new pole to the mother cell. Bud6p function has been linked to Kar9p, a protein guiding MTs along actin cables. Here, we show that Kar9p does not mediate Bud6p functions in spindle orientation. Based on live microscopy analysis, kar9Delta cells maintained Bud6p-dependent MT capture. Conversely, bud6Delta cells supported Kar9p-associated MT delivery to the bud. Moreover, additive phenotypes in bud6Delta kar9Delta or bud6Delta dyn1Delta mutants underscored the separate contributions of Bud6p, Kar9p, and dynein to spindle positioning. Finally, tub2C354S, a mutation decreasing MT dynamics, suppressed a kar9Delta mutation in a BUD6-dependent manner. Thus, Kar9p-independent capture at Bud6p sites can effect spindle orientation provided MT turnover is reduced. Together, these results demonstrate Bud6p function in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.