ArticleMolecular and cellular biology1992
Protein-DNA interactions associated with the onset of testis-specific expression of the mammalian Pgk-2 gene.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 65 citations in OpenAlex.
- Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.Reproduction (Cambridge, England) · 2013Article
- Sp1 transcription factor and GATA1 cis-acting elements modulate testis-specific expression of mouse cyclin A1.PloS one · 2012Article
- A conserved E2F6-binding element in murine meiosis-specific gene promoters.Biology of reproduction · 2008Article
- Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.Biology of reproduction · 2008Article
- In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis.Molecular and cellular biology · 2007Article
- Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq.BMC evolutionary biology · 2007Article
- Molecular characterization of the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene and its association with male fertility.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- A novel 14-base-pair regulatory element is essential for in vivo expression of murine beta4-galactosyltransferase-I in late pachytene spermatocytes and round spermatids.Molecular and cellular biology · 1999Article
- Regulation of Pdha-2 expression is mediated by proximal promoter sequences and CpG methylation.Molecular and cellular biology · 1997Article
- Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.Molecular and cellular biology · 1995Article
- A factor stimulating transcription of the testis-specific Pgk-2 gene recognizes a sequence similar to the binding site for a transcription inhibitor of the somatic-type Pgk-1 gene.Nucleic acids research · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have identified difference in protein-DNA interactions associated with the promoter of the mammalian spermatogenesis-specific Pgk-2 gene in expressing and nonexpressing cells, using a band shift assay. We compared DNA-binding activities in nuclear protein extracts from expressing adult testis cells versus nonexpressing prepuberal testis cells and nonexpressing somatic cells. One or two DNA-binding activities were found to be uniquely associated with the expressed state of Pgk-2, while a third appears to be associated with the nonexpressed state. All three of these activities map to a region within the first 40 bp upstream from the core promoter of this gene. The Pgk-2 core promoter lacks a TATA box but contain a GC box and a CAAT box. We show that the GC box binds the ubiquitous transcription factor Sp1 and that the CAAT box binds CTF-1, both of which are present in extracts from all three tissue types tested. These results suggest that tissue-specific transcription of the Pgk-2 gene is associated with changes in protein-DNA interactions occurring within a 40-bp enhancer region and that different arrays of protein-DNA interactions in this region are associated with the actively expressed state of the Pgk-2 gene in spermatocytes and spermatids and with the terminally repressed state of Pgk-2 in somatic cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.