ArticleJournal of bacteriology2004
The completely sequenced plasmid pEST4011 contains a novel IncP1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 103 citations in OpenAlex.
- Not So Mosaic After All? The Core Genomes of IncP-1 Plasmids Evolve Predominantly Through Vertical Transmission.bioRxiv : the preprint server for biology · 2025Article
- Degradation of 2,4-D by plant growth-promotingMicrobiology spectrum · 2025Article
- Microorganism-Driven 2,4-D Biodegradation: Current Status and Emerging Opportunities.Molecules (Basel, Switzerland) · 2024Review
- Evolution End Classification ofInternational journal of molecular sciences · 2023Article
- Comparative Genomic Analysis of the Regulation of Aromatic Metabolism in Betaproteobacteria.Frontiers in microbiology · 2019Article
- Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by a New Isolated Strain of Achromobacter sp. LZ35.Current microbiology · 2017Article
- Comparison of the complete genome sequences of four γ-hexachlorocyclohexane-degrading bacterial strains: insights into the evolution of bacteria able to degrade a recalcitrant man-made pesticide.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Impact of untreated urban waste on the prevalence and antibiotic resistance profiles of human opportunistic pathogens in agricultural soils from Burkina Faso.Environmental science and pollution research international · 2016Article
- Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes.FEMS microbiology ecology · 2016Article
- Complete nucleotide sequence and analysis of two conjugative broad host range plasmids from a marine microbial biofilm.PloS one · 2014Article
- Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity.The FEBS journal · 2013Article
- Quantification of IncP-1 plasmid prevalence in environmental samples.Applied and environmental microbiology · 2013Article
- Removal of alachlor in anoxic soil slurries and related alteration of the active communities.Environmental science and pollution research international · 2013Article
- Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees.Molecular biology and evolution · 2013Article
- Article
- Roles of long and short replication initiation proteins in the fate of IncP-1 plasmids.Journal of bacteriology · 2012Article
- Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.Applied and environmental microbiology · 2012Article
- IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes.Frontiers in microbiology · 2012Article
- Article
- Broad-host-range plasmids from agricultural soils have IncP-1 backbones with diverse accessory genes.Applied and environmental microbiology · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterium Achromobacter xylosoxidans subsp. denitrificans strain EST4002 contains plasmid pEST4011. This plasmid ensures its host a stable 2,4-D(+) phenotype. We determined the complete 76,958-bp nucleotide sequence of pEST4011. This plasmid is a deletion and duplication derivative of pD2M4, the 95-kb highly unstable laboratory ancestor of pEST4011, and was self-generated during different laboratory manipulations performed to increase the stability of the 2,4-D(+) phenotype of the original strain, strain D2M4(pD2M4). The 47,935-bp catabolic region of pEST4011 forms a transposon-like structure with identical copies of the hybrid insertion element IS1071::IS1471 at the two ends. The catabolic regions of pEST4011 and pJP4, the best-studied 2,4-D-degradative plasmid, both contain homologous, tfd-like genes for complete 2,4-D degradation, but they have little sequence similarity other than that. The backbone genes of pEST4011 are most similar to the corresponding genes of broad-host-range self-transmissible IncP1 plasmids. The backbones of the other three IncP1 catabolic plasmids that have been sequenced (the 2,4-D-degradative plasmid pJP4, the haloacetate-catabolic plasmid pUO1, and the atrazine-catabolic plasmid pADP-1) are nearly identical to the backbone of R751, the archetype plasmid of the IncP1 beta subgroup. We show that despite the overall similarity in plasmid organization, the pEST4011 backbone is sufficiently different (51 to 86% amino acid sequence identity between individual backbone genes) from the backbones of members of the three IncP1 subgroups (the alpha, beta, and gamma subgroups) that it belongs to a new IncP1subgroup, the delta subgroup. This conclusion was also supported by a phylogenetic analysis of the trfA2, korA, and traG gene products of different IncP1 plasmids.
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