ArticleGenome research2004
Systematic recovery and analysis of full-ORF human cDNA clones.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The completion of the Mammalian Gene Collection (MGC).Genome research · 2009Article
- Mat1 inhibits peroxisome proliferator-activated receptor gamma-mediated adipocyte differentiation.Molecular and cellular biology · 2009Article
- A biomedically enriched collection of 7000 human ORF clones.PloS one · 2008Article
- Targeted discovery of novel human exons by comparative genomics.Genome research · 2007Article
- CONTRAST: a discriminative, phylogeny-free approach to multiple informant de novo gene prediction.Genome biology · 2007Article
- LongSAGE profiling of nine human embryonic stem cell lines.Genome biology · 2007Article
- A RecA-mediated exon profiling method.Nucleic acids research · 2006Article
- Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP).Nucleic acids research · 2005Article
- A newly discovered human alpha-globin gene.Blood · 2005Article
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).Genome research · 2004Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
The Mammalian Gene Collection (MGC) consortium (http://mgc.nci.nih.gov) seeks to establish publicly available collections of full-ORF cDNAs for several organisms of significance to biomedical research, including human. To date over 15,200 human cDNA clones containing full-length open reading frames (ORFs) have been identified via systematic expressed sequence tag (EST) analysis of a diverse set of cDNA libraries; however, further systematic EST analysis is no longer an efficient method for identifying new cDNAs. As part of our involvement in the MGC program, we have developed a scalable method for targeted recovery of cDNA clones to facilitate recovery of genes absent from the MGC collection. First, cDNA is synthesized from various RNAs, followed by polymerase chain reaction (PCR) amplification of transcripts in 96-well plates using gene-specific primer pairs flanking the ORFs. Amplicons are cloned into a sequencing vector, and full-length sequences are obtained. Sequences are processed and assembled using Phred and Phrap, and analyzed using Consed and a number of bioinformatics methods we have developed. Sequences are compared with the Reference Sequence (RefSeq) database, and validation of sequence discrepancies is attempted using other sequence databases including dbEST and dbSNP. Clones with identical sequence to RefSeq or containing only validated changes will become part of the MGC human gene collection. Clones containing novel splice variants or polymorphisms have also been identified. Our approach to clone recovery, applied at large scale, has the potential to recover many and possibly most of the genes absent from the MGC collection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.