ArticleProceedings of the National Academy of Sciences of the United States of America2004
Gene targeting in yeast is initiated by two independent strand invasions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Trial
- Characterization and regulation of cell cycle-independent noncanonical gene targeting.Nature communications · 2024Article
- Gene duplication and deletion caused by over-replication at a fork barrier.Nature communications · 2023Article
- The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting inFrontiers in plant science · 2019Article
- Gene Editing with Helper-Dependent Adenovirus Can Efficiently Introduce Multiple Changes Simultaneously over a Large Genomic Region.Molecular therapy. Nucleic acids · 2017Article
- Mechanisms of precise genome editing using oligonucleotide donors.Genome research · 2017Article
- Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.Nature · 2017Article
- Mechanisms of gene targeting in higher eukaryotes.Cellular and molecular life sciences : CMLS · 2016Review
- Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection.BMC research notes · 2015Article
- Article
- Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.DNA repair · 2014Article
- RPA antagonizes microhomology-mediated repair of DNA double-strand breaks.Nature structural & molecular biology · 2014Article
- The mechanism of gene targeting in human somatic cells.PLoS genetics · 2014Article
- Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Heteroduplex formation, mismatch resolution, and genetic sectoring during homologous recombination in the hyperthermophilic archaeon sulfolobus acidocaldarius.Frontiers in microbiology · 2012Article
- Recombineering: A powerful tool for modification of bacteriophage genomes.Bacteriophage · 2012Article
- Intrachromosomal tandem duplication and repeat expansion during attempts to inactivate the subtelomeric essential gene GSH1 in Leishmania.Nucleic acids research · 2011Article
- Effects of varying gene targeting parameters on processing of recombination intermediates by ERCC1-XPF.DNA repair · 2011Article
- Single-stranded heteroduplex intermediates in lambda Red homologous recombination.BMC molecular biology · 2010Article
- DNA bridging of yeast chromosomes VIII leads to near-reciprocal translocation and loss of heterozygosity with minor cellular defects.Chromosoma · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To study the mechanism of gene targeting, we examined heteroduplex DNA (hDNA) formation during targeting of two separate chromosomal locations in Saccharomyces cerevisiae. We examined both replacement of the entire gene with a heterologous selectable marker and correction of a single base pair insertion mutation by gene targeting, and in all cases our results were consistent with separate strand invasion/resolution at the two ends of the targeting fragment as the dominant mechanism in wild-type cells. A small subset of transformants was consistent with assimilation of a single strand of targeting DNA encompassing both flanking homology regions and the marker into hDNA. hDNA formation during correction of a point mutation by targeted integration was conspicuously altered in a mismatch repair-deficient background and was consistent with single-strand invasion/assimilation without mismatch correction, confirming that gene targeting by this pathway is actively impeded in wild-type yeast. Finally, inversion of one targeted locus and mutation of an active origin of DNA replication at the other locus affected hDNA formation significantly, suggesting that formation of productive interactions between the targeting DNA and the targeted site in the chromosome is sensitive to local DNA dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.