ArticleNucleic acids research2004
Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Evaluation of the Immortalized Primary Human Hepatocyte Cell Line Fa2N-4 as a Model for Metabolic Dysfunction-Associated Steatotic Liver Disease.Gastro hep advances · 2026Article
- Synthetic rewiring and boosting type I interferon responses for visualization and counteracting viral infections.Nucleic acids research · 2020Article
- Murine Cytomegalovirus M25 Proteins Sequester the Tumor Suppressor Protein p53 in Nuclear Accumulations.Journal of virology · 2020Article
- Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor.PLoS pathogens · 2018Article
- Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3European journal of immunology · 2017Article
- The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.Scientific reports · 2017Article
- Proliferation status defines functional properties of endothelial cells.Cellular and molecular life sciences : CMLS · 2017Article
- Immortalized Human Schwann Cell Lines Derived From Tumors of Schwannomatosis Patients.PloS one · 2015Article
- Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Experimental design to evaluate directed adaptive mutation in Mammalian cells.JMIR research protocols · 2014Article
- Strategies for immortalization of primary hepatocytes.Journal of hepatology · 2014Review
- Mouse cytomegalovirus infection overrules T regulatory cell suppression on natural killer cells.Virology journal · 2014Article
- A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells.Virology journal · 2013Article
- RNA-binding protein HuR autoregulates its expression by promoting alternative polyadenylation site usage.Nucleic acids research · 2012Article
- Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.Nucleic acids research · 2011Article
- Alternatives to animal experimentation for hormonal compounds research.Genes & nutrition · 2009Article
- Development of an immortalised, post-pubertal gonadotrophin-releasing hormone neuronal cell line.Journal of neuroendocrinology · 2008Article
- Current status of transcriptional regulation systems.Cytotechnology · 2006Article
- Application of a reversible immortalization system for the generation of proliferation-controlled cell lines.Cytotechnology · 2004Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conditional proliferation of mouse embryo fibroblasts was achieved with a novel autoregulatory vector for Tet-dependent expression of the SV40 T-antigen. The majority of cell clones that were isolated under induced conditions showed strict regulation of cell growth. Status switches were found to be fully reversible and highly reproducible with respect to gene expression characteristics. A consequence of T-antigen expression is a significant deregulation of >400 genes. Deinduced cells turn to rest in G0/G1 phase and exhibit a senescent phenotype. The cells are not oncogenic and no evidence for transformation was found after several months of cultivation. Conditional immortalization allows diverse studies including those on cellular activities without the influence of the immortalizing gene(s), senescence as well as secondary effects from T-antigen expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.