ArticleThe Plant cell2004
Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 170 papers.
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Who cites it
170 citing papers in PubMed.
- Actin Isovariant ACT2-Mediated Cellular Auxin Homeostasis Regulates Lateral Root Organogenesis in Arabidopsis thaliana.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Dual ubiquitin signalling by SLOMO controls AUX1 activity and turnover during root gravitropism.The EMBO journal · 2026Article
- RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development.Nature plants · 2025Article
- Structural basis of auxin binding and transport byProceedings of the National Academy of Sciences of the United States of America · 2025Article
- ThePlants (Basel, Switzerland) · 2025Article
- Auxin Controls Root Gravitropic Response by Affecting Starch Granule Accumulation and Cell Wall Modification in Tomato.Plants (Basel, Switzerland) · 2025Article
- Review
- Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation.Science advances · 2025Article
- CUC/auxin patterning of decanalised petal number inQuantitative plant biology · 2025Article
- TYPHON proteins are RAB-dependent mediators of the trans-Golgi network secretory pathway.The Plant cell · 2024Article
- Comprehensive Transcriptomic and Physiological Insights into the Response of Root Growth Dynamics During the Germination of Diverse Sesame Varieties to Heat Stress.Current issues in molecular biology · 2024Article
- Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress.Plants (Basel, Switzerland) · 2024Review
- Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology.Plant methods · 2024Article
- A root cap-localized NAC transcription factor controls root halotropic response to salt stress in Arabidopsis.Nature communications · 2024Article
- Genome-wide identification and comparative analysis of the Amino Acid Transporter (AAT) gene family and their roles during Phaseolus vulgaris symbioses.Functional & integrative genomics · 2024Article
- Visualizing Orientation and Topology of ER Membrane Proteins In Planta.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Physiological and transcriptomic analysis reveals the potential mechanism of Morinda officinalis How in response to freezing stress.BMC plant biology · 2023Article
- Article
- Lysinibacillus spp.: an IAA-producing endospore forming-bacteria that promotes plant growth.Antonie van Leeuwenhoek · 2023Article
- Dynamic GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in the root cap mediates root gravitropism.Plant physiology · 2023Article
110 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
Abstract
We have investigated the subcellular localization, the domain topology, and the amino acid residues that are critical for the function of the presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments and confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed that AUX1 colocalized with plasma membrane (PM) markers. Because of its PM localization, we were able to take advantage of the steep pH gradient that exists across the plant cell PM to investigate AUX1 topology using YFP as a pH-sensitive probe. The YFP-coding sequence was inserted in selected AUX1 hydrophilic loops to orient surface domains on either apoplastic or cytoplasmic faces of the PM based on the absence or presence of YFP fluorescence, respectively. We were able to demonstrate in conjunction with helix prediction programs that AUX1 represents a polytopic membrane protein composed of 11 transmembrane spanning domains. In parallel, a large aux1 allelic series containing null, partial-loss-of-function, and conditional mutations was characterized to identify the functionally important domains and amino acid residues within the AUX1 polypeptide. Whereas almost all partial-loss-of-function and null alleles cluster in the core permease region, the sole conditional allele aux1-7 modifies the function of the external C-terminal domain.
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