ArticleThe Plant cell2004
Novel targeting signals mediate the sorting of different isoforms of the tail-anchored membrane protein cytochrome b5 to either endoplasmic reticulum or mitochondria.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 106 citations in OpenAlex.
- A plant-specific cytochromeScience advances · 2025Article
- Cytochrome b5 diversity in green lineages preceded the evolution of syringyl lignin biosynthesis.The Plant cell · 2024Article
- A distinct dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo.BMC biology · 2024Article
- Tissue-preferential recruitment of electron transfer chains for cytochrome P450-catalyzed phenolic biosynthesis.Science advances · 2023Article
- CytochromeFrontiers in plant science · 2022Review
- An endoplasmic reticulum-localized cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochromePlant signaling & behavior · 2020Article
- Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas.BMC genomics · 2020Article
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses.Plant physiology · 2020Article
- Biogenesis of chloroplast outer envelope membrane proteins.Plant cell reports · 2019Review
- CytochromeThe Plant cell · 2019Article
- Membrane-Specific Targeting of Tail-Anchored Proteins SECE1 and SECE2 Within Chloroplasts.Frontiers in plant science · 2019Article
- Analysis of tail-anchored protein translocation pathway in plants.Biochemistry and biophysics reports · 2018Article
- Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.Annual review of cell and developmental biology · 2017Review
- Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants.Plant biotechnology journal · 2017Article
- Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii.Traffic (Copenhagen, Denmark) · 2017Article
- Involvement of Iron-Containing Proteins in Genome Integrity in Arabidopsis Thaliana.Genome integrity · 2015Review
- Identification of unique SUN-interacting nuclear envelope proteins with diverse functions in plants.The Journal of cell biology · 2014Article
- Giardia intestinalis incorporates heme into cytosolic cytochrome b₅.Eukaryotic cell · 2014Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tail-anchored membrane proteins are a class of proteins that are targeted posttranslationally to various organelles and integrated by a single segment of hydrophobic amino acids located near the C terminus. Although the localization of tail-anchored proteins in specific subcellular compartments in plant cells is essential for their biological function, the molecular targeting signals responsible for sorting these proteins are not well defined. Here, we describe the biogenesis of four closely related tung (Aleurites fordii) cytochrome b5 isoforms (Cb5-A, -B, -C, and -D), which are small tail-anchored proteins that play an essential role in many cellular processes, including lipid biosynthesis. Using a combination of in vivo and in vitro assays, we show that Cb5-A, -B, and -C are targeted exclusively to the endoplasmic reticulum (ER), whereas Cb5-D is targeted specifically to mitochondrial outer membranes. Comprehensive mutational analyses of ER and mitochondrial Cb5s revealed that their C termini, including transmembrane domains (TMD) and tail regions, contained several unique physicochemical and sequence-specific characteristics that defined organelle-specific targeting motifs. Mitochondrial targeting of Cb5 was mediated by a combination of hydrophilic amino acids along one face of the TMD, an enrichment of branched beta-carbon-containing residues in the medial portion of the TMD, and a dibasic -R-R/K/H-x motif in the C-terminal tail. By contrast, ER targeting of Cb5 depended primarily upon the overall length and hydrophobicity of the TMD, although an -R/H-x-Y/F- motif in the tail was also a targeting determinant. Collectively, the results presented provide significant insight into the early biogenetic events required for entry of tail-anchored proteins into either the ER or mitochondrial targeting pathways.
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