ArticleMolecular and cellular biology2004
A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- The emerging role of SARS-CoV-2 nonstructural protein 1 (nsp1) in epigenetic regulation of host gene expression.FEMS microbiology reviews · 2024Review
- Single-molecule characterization of SV40 replisome and novel factors: human FPC and Mcm10.Nucleic acids research · 2024Article
- Article
- Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- AND-1 fork protection function prevents fork resection and is essential for proliferation.Nature communications · 2018Article
- Level of FACT defines the transcriptional landscape and aggressive phenotype of breast cancer cells.Oncotarget · 2017Article
- Chromosome Duplication in Saccharomyces cerevisiae.Genetics · 2016Review
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- Curaxin CBL0137 eradicates drug resistant cancer stem cells and potentiates efficacy of gemcitabine in preclinical models of pancreatic cancer.Oncotarget · 2014Article
- The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.Cell division · 2014Article
- Complex mutual regulation of facilitates chromatin transcription (FACT) subunits on both mRNA and protein levels in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Facilitates chromatin transcription complex is an "accelerator" of tumor transformation and potential marker and target of aggressive cancers.Cell reports · 2013Article
- An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae.Chromosoma · 2013Article
- The role of FACT in making and breaking nucleosomes.Biochimica et biophysica acta · 2012Article
- Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication.Biochimica et biophysica acta · 2011Article
- Replication-compromised cells require the mitotic checkpoint to prevent tetraploidization.Chromosoma · 2011Article
- Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-replication factor C (RFC) and DNA polymerase {epsilon} is mediated by the cohesion-specific subunits, Ctf18, Dcc1, and Ctf8.The Journal of biological chemistry · 2010Article
- Differences in the DNA replication of unicellular eukaryotes and metazoans: known unknowns.EMBO reports · 2010Review
- Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication.Journal of virology · 2010Article
- Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesion.The EMBO journal · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
DNA replication depends critically upon chromatin structure. Little is known about how the replication complex overcomes the nucleosome packages in chromatin during DNA replication. To address this question, we investigate factors that interact in vivo with the principal initiation DNA polymerase, DNA polymerase alpha (Polalpha). The catalytic subunit of budding yeast Polalpha (Pol1p) has been shown to associate in vitro with the Spt16p-Pob3p complex, a component of the nucleosome reorganization system required for both replication and transcription, and with a sister chromatid cohesion factor, Ctf4p. Here, we show that an N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo. A mutation in a glycine residue in this N-terminal region of POL1 compromises the ability of Pol1p to associate with Spt16p and alters the temporal ordered association of Ctf4p with Pol1p. The compromised association between the chromatin-reorganizing factor Spt16p and the initiating DNA polymerase Pol1p delays the Pol1p assembling onto and disassembling from the late-replicating origins and causes a slowdown of S-phase progression. Our results thus suggest that a coordinated temporal and spatial interplay between the conserved N-terminal region of the Polalpha protein and factors that are involved in reorganization of nucleosomes and promoting establishment of sister chromatin cohesion is required to facilitate S-phase progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.