ArticleJournal of medical virology2004
Development and evaluation of a real-time nucleic acid sequence based amplification assay for rapid detection of influenza A.
Article in Journal of medical virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 81 citations in OpenAlex.
- Spherical Nucleic Acid Stabilized Cage Type Three-Dimensional Electrochemiluminescence Probe for Sensitive and Amplification-Free Diagnosis of Infectious Diseases.Exploration (Beijing, China) · 2026Article
- Advances in Diagnostic Techniques for Influenza Virus Infection: A Comprehensive Review.Tropical medicine and infectious disease · 2025Review
- Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections.ACS omega · 2022Article
- Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza.Biosensors · 2021Review
- Graphene functionalized field-effect transistors for ultrasensitive detection of Japanese encephalitis and Avian influenza virus.Scientific reports · 2020Article
- Development of a real-time nucleic acid sequence-based amplification assay for the rapid detection of Salmonella spp. from food.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Review
- Review
- Evolving gene targets and technology in influenza detection.Molecular diagnosis & therapy · 2013Review
- Alternative molecular tests for virological diagnosis.Molecular biotechnology · 2013Review
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- Automated extraction and amplification for direct detection of Mycobacterium tuberculosis complex in various clinical samples.Journal of clinical microbiology · 2011Article
- Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.BMC infectious diseases · 2010Article
- Adamantane resistance in seasonal human influenza A viruses from Calgary, Alberta (January 2007 to August 2008).The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2010Article
- Enhanced viral etiological diagnosis of respiratory system infection outbreaks by use of a multitarget nucleic acid amplification assay.Journal of clinical microbiology · 2009Article
- Utilization of nucleic acid amplification assays for the detection of respiratory viruses.Clinics in laboratory medicine · 2009Review
- Design and validation of real-time reverse transcription-PCR assays for detection of pandemic (H1N1) 2009 virus.Journal of clinical microbiology · 2009Article
- design of multiplexed detection assays for identification of avian influenza a virus subtypes pathogenic to humans by SmartCycler real-time reverse transcription-PCR.Journal of clinical microbiology · 2009Article
- Detection of respiratory viruses by molecular methods.Clinical microbiology reviews · 2008Review
- Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections.Journal of clinical microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development and introduction of effective treatment for influenza A in the form of neuraminidase inhibitors have made the rapid diagnosis of infection important especially in high-risk populations. The aim of this study was to develop a real-time nucleic acid sequenced based amplification (NASBA) using a molecular beacon that could detect a wide range of influenza A subtypes and strains in a single reaction by targeting a conserved region of the influenza genome, and to evaluate its sensitivity and specificity against traditional laboratory techniques on a range of clinical samples usefulness during the 2003/2004 influenza season. The results demonstrated the assay to be highly sensitive and specific, detecting <0.1 TCID50 of virus stock. Three hundred eighty-nine clinical samples were tested in total from two patient groups. Overall, the real-time NASBA assay detected 64% (66/103) more influenza positive samples than cell culture and direct immunofluorescence (IF) and, therefore, was shown to be more sensitive in detecting influenza A in a wide range of respiratory samples than traditional methods. In conclusion, the real-time influenza A assay demonstrated clinical usefulness in both hospital and community populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.