ArticleMolecular biology of the cell2004
Dictyostelium PAKc is required for proper chemotaxis.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Convergence of Ras- and Rac-regulated formin pathways is pivotal for phagosome formation and particle uptake inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cdc42/Rac Interactive Binding Containing Effector Proteins in Unicellular Protozoans With Reference to Human Host: Locks of the Rho Signaling.Frontiers in genetics · 2022Review
- Review
- Function of small GTPases in Dictyostelium macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- PakD, a putative p21-activated protein kinase in Dictyostelium discoideum, regulates actin.Eukaryotic cell · 2014Article
- Article
- PakB binds to the SH3 domain of Dictyostelium Abp1 and regulates its effects on cell polarity and early development.Molecular biology of the cell · 2013Article
- Actin cytoskeleton of chemotactic amoebae operates close to the onset of oscillations.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PAK family kinases: Physiological roles and regulation.Cellular logistics · 2012Article
- Article
- Signaling mechanisms for chemotaxis.Development, growth & differentiation · 2011Review
- A new Rac/PAK/GC/cGMP signaling pathway.Molecular and cellular biochemistry · 2010Review
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity.Annual review of biophysics · 2010Review
- The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum.Cell motility and the cytoskeleton · 2009Article
- PAK thread from amoeba to mammals.Journal of cellular biochemistry · 2009Review
- Myosin IIA associates with NK cell lytic granules to enable their interaction with F-actin and function at the immunological synapse.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Localized activation of p21-activated kinase controls neuronal polarity and morphology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Big roles for small GTPases in the control of directed cell movement.The Biochemical journal · 2007Review
- RacG regulates morphology, phagocytosis, and chemotaxis.Eukaryotic cell · 2006Article
- Positive feedback may cause the biphasic response observed in the chemoattractant-induced response of Dictyostelium cells.Systems & control letters · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a new Dictyostelium p21-activated protein kinase, PAKc, that we demonstrate to be required for proper chemotaxis. PAKc contains a Rac-GTPase binding (CRIB) and autoinhibitory domain, a PAK-related kinase domain, an N-terminal phosphatidylinositol binding domain, and a C-terminal extension related to the Gbetagamma binding domain of Saccharomyces cerevisiae Ste20, the latter two domains being required for PAKc transient localization to the plasma membrane. In response to chemoattractant stimulation, PAKc kinase activity is rapidly and transiently activated, with activity levels peaking at approximately 10 s. pakc null cells exhibit a loss of polarity and produce multiple lateral pseudopodia when placed in a chemoattractant gradient. PAKc preferentially binds the Dictyostelium Rac protein RacB, and point mutations in the conserved CRIB that abrogate this binding result in misregulated kinase activation and chemotaxis defects. We also demonstrate that a null mutation lacking the PAK family member myosin I heavy chain kinase (MIHCK) shows mild chemotaxis defects, including the formation of lateral pseudopodia. A null strain lacking both PAKc and the PAK family member MIHCK exhibits severe loss of cell movement, suggesting that PAKc and MIHCK may cooperate to regulate a common chemotaxis pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.