ArticleMolecular biology of the cell2005
BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Schizosaccharomyces pombe KAT5 contributes to resection and repair of a DNA double-strand break.Genetics · 2021Article
- Article
- Plant centromeres: genetics, epigenetics and evolution.Molecular biology reports · 2018Review
- Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation.The Journal of biological chemistry · 2015Article
- Actin complexes in the cell nucleus: new stones in an old field.Histochemistry and cell biology · 2010Review
- The fission yeast inhibitor of growth (ING) protein Png1p functions in response to DNA damage.The Journal of biological chemistry · 2010Article
- Chapter 5. Nuclear actin-related proteins in epigenetic control.International review of cell and molecular biology · 2009Review
- Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast.Nature structural & molecular biology · 2008Article
- Article
- Forward genetic analysis of the apicomplexan cell division cycle in Toxoplasma gondii.PLoS pathogens · 2008Article
- Gcn5p plays an important role in centromere kinetochore function in budding yeast.Molecular and cellular biology · 2008Article
- Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.Genome biology · 2008Article
- Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells.Molecular biology of the cell · 2007Article
- Article
- Actin-related protein Arp4 functions in kinetochore assembly.Nucleic acids research · 2007Article
- Sequence and comparative genomic analysis of actin-related proteins.Molecular biology of the cell · 2005Article
- Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.Molecular and cellular biology · 2005Article
- The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repression of flowering in Arabidopsis.The Plant cell · 2005Article
- Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.Molecular biology of the cell · 2005Article
- The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.Nucleic acids research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear actin-related proteins play vital roles in transcriptional regulation; however, their biological roles remain elusive. Here, we characterize Alp5, fission yeast homolog of Arp4/BAF53. The temperature-sensitive mutant alp5-1134 contains a single amino acid substitution in the conserved C-terminal domain (S402N) and displays mitotic phenotypes, including chromosome condensation and missegregation. Alp5 forms a complex with Mst1-HAT (histone acetyltransferase). Consistently, inhibition of histone deacetylases (HDACs), by either addition of a specific inhibitor or a mutation in HDAC-encoding clr6+ gene, rescues alp5-1134. Immunoblotting with specific antibodies against acetylated histones shows that Alp5 is required for histone H4 acetylation at lysines 5, 8, and 12, but not histone H3 lysines 9 or 14, and furthermore Clr6 plays an opposing role. Mitotic arrest is ascribable to activation of the Mad2/Bub1 spindle checkpoint, in which both proteins localize to the mitotic kinetochores in alp5-1134. Intriguingly, alp5-1134 displays transcriptional desilencing at the core centromere without altering the overall chromatin structure, which also is suppressed by a simultaneous mutation in clr6+. This result shows that Alp5 is essential for histone H4 acetylation, and its crucial role lies in the establishment of bipolar attachment of the kinetochore to the spindle and transcriptional silencing at the centromere.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.