ArticleExtremophiles : life under extreme conditions2004
Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus.
Article in Extremophiles : life under extreme conditions, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Comparative Analysis ofInternational journal of molecular sciences · 2022Article
- Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase.Microbial cell factories · 2021Article
- Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications.World journal of microbiology & biotechnology · 2019Review
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Molecular biology of fuselloviruses and their satellites.Extremophiles : life under extreme conditions · 2014Review
- DNA replication and strand asymmetry in prokaryotic and mitochondrial genomes.Current genomics · 2012Article
- Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.Applied and environmental microbiology · 2011Article
- A large scale comparative genomic analysis reveals insertion sites for newly acquired genomic islands in bacterial genomes.BMC microbiology · 2011Article
- Hot transcriptomics.Archaea (Vancouver, B.C.) · 2011Article
- Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance.Journal of bacteriology · 2007Article
- Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea.Nucleic acids research · 2007Article
- Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus sp.Nucleic acids research · 2007Article
- Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus.Extremophiles : life under extreme conditions · 2006Review
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6 authors.
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Abstract
Here, we describe the identification of a chromosomal DNA replication origin (oriC) from the hyperthermophilic archaeon Sulfolobus solfataricus (subdomain of Crenarchaeota). By means of a cumulative GC-skew analysis of the Sulfolobus genome sequence, a candidate oriC was mapped within a 1.12-kb region located between the two divergently transcribed MCM- and cdc6-like genes. We demonstrated that plasmids containing the Sulfolobus oriC sequence and a hygromycin-resistance selectable marker were maintained in an episomal state in transformed S. solfataricus cells under selective pressure. The proposed location of the origin was confirmed by 2-D gel electrophoresis experiments. This is the first report on the functional cloning of a chromosomal oriC from an archaeon and represents an important step toward the reconstitution of an archaeal in vitro DNA replication system.
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