ArticlePlant cell reports2005
Utility of the FLP-FRT recombination system for genetic manipulation of rice.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Selectable Markers to Marker-Free Selection in Rice.Molecular biotechnology · 2022Review
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae.Microbial cell factories · 2015Article
- Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice.Molecular biotechnology · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- A vector set for systematic metabolic engineering in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2011Article
- ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.Transgenic research · 2009Article
- Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants.Plant cell reports · 2008Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
- Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice.Nucleic acids research · 2007Article
- Site-specific DNA excision in transgenic rice with a cell-permeable cre recombinase.Molecular biotechnology · 2006Article
- Site-specific recombination in Zea mays.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To develop an FLP-FRT recombination system- (derived from 2 mu plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the beta-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
Indexed as
Identifiers
15480685What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.