ArticleJournal of virology2004
Membrane binding properties and terminal residues of the mature hepatitis C virus capsid protein in insect cells.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Structure and function of the RNA polymerase complex of Borna disease virus, a nuclear-replicating non-segmented negative-strand RNA virus.Nucleic acids research · 2026Article
- Vesiculopolins, a New Class of Anti-Vesiculoviral Compounds, Inhibit Transcription Initiation of Vesiculoviruses.Viruses · 2019Article
- TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis.Nature communications · 2016Article
- Hepatitis C virus and host cell nuclear transport machinery: a clandestine affair.Frontiers in microbiology · 2015Review
- Hallmarks of hepatitis C virus in equine hepacivirus.Journal of virology · 2014Article
- Production and pathogenicity of hepatitis C virus core gene products.World journal of gastroenterology · 2014Review
- In vitro capping and transcription of rhabdoviruses.Methods (San Diego, Calif.) · 2013Review
- Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein.FEBS letters · 2012Article
- Structural analysis of hepatitis C virus core-E1 signal peptide and requirements for cleavage of the genotype 3a signal sequence by signal peptide peptidase.Journal of virology · 2012Article
- Morphogenesis of infectious hepatitis C virus particles.Frontiers in microbiology · 2012Article
- Exploitation of lipid components by viral and host proteins for hepatitis C virus infection.Frontiers in microbiology · 2012Article
- Article
- Identification of basic amino acids at the N-terminal end of the core protein that are crucial for hepatitis C virus infectivity.Journal of virology · 2010Article
- Lipid metabolism and HCV infection.Viruses · 2010Article
- Genetic analysis of the carboxy-terminal region of the hepatitis C virus core protein.Journal of virology · 2010Article
- Palmitoylation of hepatitis C virus core protein is important for virion production.The Journal of biological chemistry · 2009Article
- Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation.Journal of virology · 2008Article
- A method for in vitro assembly of hepatitis C virus core protein and for screening of inhibitors.Analytical biochemistry · 2007Article
- Core protein domains involved in hepatitis C virus-like particle assembly and budding at the endoplasmic reticulum membrane.Cellular microbiology · 2007Article
- E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis C virus core protein.Journal of virology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immature core protein (p23, residues 1 to 191) of hepatitis C virus undergoes posttranslational modifications including intramembranous proteolysis within its C-terminal signal sequence by signal peptide peptidase to generate the mature form (p21). In this study, we analyzed the cleavage site and other amino acid modifications that occur on the core protein. To produce the posttranslationally modified core protein, we used a baculovirus-insect cell expression model system. As previously reported, p23 is processed to form p21 in insect as well as in mammalian cells. p21 was found to be associated with the cytoplasmic membrane, and its significant portion behaved as an integral membrane protein. The protein was purified from the membrane by a simple and unique procedure on the basis of its membrane-binding properties and solubility in detergents. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of purified p21 showed that the average molecular mass (m/z 19,307) of its single-charged ion differs by m/z 1,457 from that calculated for p23. To determine the posttranslational modifications, tryptic p21 peptides were analyzed by MALDI-TOF MS. We found three peptides that did not match the theoretically derived peptides of p23. Analysis of these peptides by MALDI-TOF tandem MS revealed that they correspond to N-terminal peptides (residues 2 to 9 and 2 to 10) starting with alpha-N-acetylserine and C-terminal peptide (residues 150 to 177) ending with phenylalanine. These results suggest that the mature core protein (molecular mass of 19,306 Da) includes residues 2 to 177 and that its N terminus is blocked with an acetyl group.
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