ReviewNucleic acids research2004
'Conserved hypothetical' proteins: prioritization of targets for experimental study.
Review in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 224 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
224 citing papers in PubMed, 410 citations in OpenAlex.
- Functional Annotation Identifies 3-Oxoacyl Synthase III as a Druggable Target in Nocardia asteroides FDAARGOS1485: A Multi-Tier Structure-Guided Computational Discovery of Marine-Derived Therapeutic Leads.Applied biochemistry and biotechnology · 2026Article
- Characterization of hypothetical proteins reveals function modules and virulence factors in Pseudomonas aeruginosa.Nature communications · 2026Article
- A microbiome meta-transcriptomics pipeline identifies a neutrophil elastase inhibitor that protects the colonic epithelial barrier.Signal transduction and targeted therapy · 2026Article
- Oxidative stress-triggered UMPylation of SodA by YdiU modulates oxidative stress resistance in Salmonella.Veterinary research · 2026Article
- Anticodon loop remodeling and D-stem shape drive the specific recognition of ANN-decoding tRNAs for t6A modification.Nucleic acids research · 2026Article
- Gene presence-absence and evolutionary signals of positive selection associated with virulence divergence inMicrobial genomics · 2026Article
- Harnessing interpretable deep learning to predict resistance inFrontiers in cellular and infection microbiology · 2026Article
- Recent Characterization of Key Proteins to Combat Tuberculosis.Advances in experimental medicine and biology · 2026Review
- Bioinformatics Approaches for Functional and Structural Annotation and Molecular Docking Study of a Hypothetical Protein From Staphylococcus aureus.BioMed research international · 2026Article
- Functional Annotation and Molecular Characterization of a Hypothetical Protein FromEvolutionary bioinformatics online · 2026Article
- Prediction of virulence factors in bacterial hypothetical proteins.Archives of microbiology · 2025Article
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- Comparative proteomic analysis to annotate the structural association of the hypothetical proteins from the conserved domain of P. aeruginosa as novel vaccine candidates.Biotechnology letters · 2024Article
- OSGEP regulates islet β-cell function by modulating proinsulin translation and maintaining ER stress homeostasis in mice.Nature communications · 2024Article
- Leptospira interrogans encodes a canonical BamA and three novel noNterm Omp85 outer membrane protein paralogs.Scientific reports · 2024Article
- Cellular-resolution gene expression mapping reveals organization in the head ganglia of the gastropod, Berghia stephanieae.The Journal of comparative neurology · 2024Article
- Improved prediction of DNA and RNA binding proteins with deep learning models.Briefings in bioinformatics · 2024Article
- Potential involvement of beta-lactamase homologous proteins in resistance to beta-lactam antibiotics in gram-negative bacteria of the ESKAPEE group.BMC genomics · 2024Article
- Genetic Analysis of the ts-Lethal MutantGenes · 2024Article
164 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Comparative genomics shows that a substantial fraction of the genes in sequenced genomes encodes 'conserved hypothetical' proteins, i.e. those that are found in organisms from several phylogenetic lineages but have not been functionally characterized. Here, we briefly discuss recent progress in functional characterization of prokaryotic 'conserved hypothetical' proteins and the possible criteria for prioritizing targets for experimental study. Based on these criteria, the chief one being wide phyletic spread, we offer two 'top 10' lists of highly attractive targets. The first list consists of proteins for which biochemical activity could be predicted with reasonable confidence but the biological function was predicted only in general terms, if at all ('known unknowns'). The second list includes proteins for which there is no prediction of biochemical activity, even if, for some, general biological clues exist ('unknown unknowns'). The experimental characterization of these and other 'conserved hypothetical' proteins is expected to reveal new, crucial aspects of microbial biology and could also lead to better functional prediction for medically relevant human homologs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.