ArticleThe EMBO journal1992
Ribosomal frameshifting in plants: a novel signal directs the -1 frameshift in the synthesis of the putative viral replicase of potato leafroll luteovirus.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 87 citations in OpenAlex.
- Identification of a Novel Polerovirus in Cocoa (Pathogens (Basel, Switzerland) · 2023Article
- Polerovirus genomic variation.Virus evolution · 2021Article
- Identification, Characterization and Full-Length Sequence Analysis of a Novel Polerovirus Associated with Wheat Leaf Yellowing Disease.Frontiers in microbiology · 2017Article
- Article
- The complete nucleotide sequence of the genome of Barley yellow dwarf virus-RMV reveals it to be a new Polerovirus distantly related to other yellow dwarf viruses.Frontiers in microbiology · 2013Article
- Virus versus host cell translation love and hate stories.Advances in virus research · 2009Review
- A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment.Molecular cell · 2006Article
- Characterization of the frameshift signal of Edr, a mammalian example of programmed -1 ribosomal frameshifting.Nucleic acids research · 2005Article
- Factors affecting translation at the programmed -1 ribosomal frameshifting site of Cocksfoot mottle virus RNA in vivo.Nucleic acids research · 2005Article
- An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Structural analysis of the -1 ribosomal frameshift elements in giardiavirus mRNA.Journal of virology · 2001Article
- Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA.Journal of virology · 2000Article
- Article
- Mutational study reveals that tertiary interactions are conserved in ribosomal frameshifting pseudoknots of two luteoviruses.RNA (New York, N.Y.) · 2000Article
- RNA structure and the regulation of gene expression.Plant molecular biology · 1996Review
- Translation in plants--rules and exceptions.Plant molecular biology · 1996Review
- Gene expression from viral RNA genomes.Plant molecular biology · 1996Review
- Programmed translational frameshifting.Microbiological reviews · 1996Review
- Characterization of coronavirus RNA polymerase gene products.Methods in enzymology · 1996Article
- Principles of molecular organization, expression, and evolution of closteroviruses: over the barriers.Advances in virus research · 1996Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5.8 kb RNA genome of potato leafroll luteovirus (PLRV) contains two overlapping open reading frames, ORF2a and ORF2b, which are characterized by helicase and RNA polymerase motifs, respectively, and possibly represent the viral replicase. Within the overlap, ORF2b lacks an AUG translational start codon and is therefore presumably translated by -1 ribosomal frameshifting as a transframe protein with ORF2a. This hypothesis was studied by introducing the putative frameshift region into an internal position of the beta-glucuronidase (GUS) gene and testing for the occurrence of frameshifting in vivo by transient expression of GUS activity in potato protoplasts as well as in vitro by translation in the reticulocyte system. Both experimental approaches demonstrate that a -1 frameshift occurs at a frequency of approximately 1%. Site-directed mutagenesis identified the frameshift region and the involvement of the novel heptanucleotide motif UUUAAAU in conjunction with an adjacent stem-loop structure. Part of this stem-loop encodes a basic region in the ORF2b moiety of the transframe protein which was shown by binding experiments with PLRV RNA to represent a nucleic acid-binding domain. These data support a possible biological significance of the frameshift to occur at this position of the large overlap by including the putative RNA template-binding site of the PLRV replicase in the ORF2a/ORF2b transframe protein.
Indexed as
Identifiers
1547775PMC556553W1823867477What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.