ArticleNucleic acids research2004
A high resolution protein interaction map of the yeast Mediator complex.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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Who cites it
117 citing papers in PubMed.
- The Mediator Kinase Module regulates cell cycle re-entry and transcriptional responses following DNA damage.bioRxiv : the preprint server for biology · 2025Article
- Identifying associations between short tandem repeat sequences and gene expression in yeast reveals specific repeated motifs encoding transcriptional regulatory proteins.Computational and structural biotechnology journal · 2025Article
- MEDIATOR SUBUNIT17 is required for transcriptional optimization of root system architecture in Arabidopsis.Plant physiology · 2023Article
- MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis.Plant physiology · 2022Article
- Mediator Complex of the Malaria ParasiteACS omega · 2022Article
- Stimulation of adaptive gene amplification by origin firing under replication fork constraint.Nucleic acids research · 2022Article
- Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.eLife · 2021Article
- Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network.PLoS genetics · 2021Article
- Identification of theFrontiers in plant science · 2021Article
- MedProDB: A database of Mediator proteins.Computational and structural biotechnology journal · 2021Article
- Possible Role for Allelic Variation in YeastFrontiers in microbiology · 2021Article
- Interaction map of Arabidopsis Mediator complex expounding its topology.Nucleic acids research · 2019Article
- Role of integrative structural biology in understanding transcriptional initiation.Methods (San Diego, Calif.) · 2019Review
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- Article
- Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative.Nature communications · 2018Article
- Modeling the assembly order of multimeric heteroprotein complexes.PLoS computational biology · 2018Article
- A Cotton Cyclin-Dependent Kinase E Confers Resistance toFrontiers in plant science · 2018Article
- ScMED7, a sugarcane mediator subunit gene, acts as a regulator of plant immunity and is responsive to diverse stress and hormone treatments.Molecular genetics and genomics : MGG · 2017Article
- A pathway for low zinc homeostasis that is conserved in animals and acts in parallel to the pathway for high zinc homeostasis.Nucleic acids research · 2017Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator is a large, modular protein complex remotely conserved from yeast to man that conveys regulatory signals from DNA-binding transcription factors to RNA polymerase II. In Saccharomyces cerevisiae, Mediator is thought to be composed of 24 subunits organized in four sub-complexes, termed the head, middle, tail and Cdk8 (Srb8-11) modules. In this work, we have used screening and pair-wise two-hybrid approaches to investigate protein-protein contacts between budding yeast Mediator subunits. The derived interaction map includes the delineation of numerous interaction domains between Mediator subunits, frequently corresponding to segments that have been conserved in evolution, as well as novel connections between the Cdk8 (Srb8-11) and head modules, the head and middle modules, and the middle and tail modules. The two-hybrid analysis, together with co-immunoprecipitation studies and gel filtration experiments revealed that Med31 (Soh1) is associated with the yeast Mediator that therefore comprises 25 subunits. Finally, analysis of the protein interaction network within the Drosophila Mediator middle module indicated that the structural organization of the Mediator complex is conserved from yeast to metazoans. The resulting interaction map provides a framework for delineating Mediator structure-function and investigating how Mediator function is regulated.
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