ArticlePlant physiology2004
Arabidopsis HMA2, a divalent heavy metal-transporting P(IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 233 citations in OpenAlex.
- Structural and functional insights into a ZnNature communications · 2026Article
- CRISPR/Cas9-mediated knockout of ZmHMA3 reveals its essential role in zinc homeostasis and high-zinc stress tolerance in maize.Scientific reports · 2026Article
- ZnO and rGO-Based nanoparticles regulate morphophysiological, biochemical, and molecular responses of Phaseolus vulgaris L. under drought and arsenic stress.BMC plant biology · 2026Article
- High-permeability cellulose nanocrystals mediate systemic zinc redistribution through nsLTP2-dependent immune potentiation in plants.Plant biotechnology journal · 2025Article
- Combating plant diseases through transition metal allocation.The New phytologist · 2025Review
- Arabidopsis thaliana Zn transporter genes ZIP3 and ZIP5 provide the main Zn uptake route and act redundantly to face Zn deficiency.The Plant journal : for cell and molecular biology · 2025Article
- Cadmium Stress Signaling Pathways in Plants: Molecular Responses and Mechanisms.Current issues in molecular biology · 2024Review
- Phytoremediation potential of Brassica oleracea varieties through cadmium tolerance gene expression analysis.Journal, genetic engineering & biotechnology · 2024Article
- The Uptake, Transfer, and Detoxification of Cadmium in Plants and Its Exogenous Effects.Cells · 2024Review
- Overexpression ofPlants (Basel, Switzerland) · 2023Article
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- Genetic assimilation of ancestral plasticity during parallel adaptation to zinc contamination in Silene uniflora.Nature ecology & evolution · 2023Article
- Ectopic Expression ofInternational journal of molecular sciences · 2023Article
- A dynamic compartment model for xylem loading and long-distance transport of iron explains the effect of kanamycin on metal uptake inFrontiers in plant science · 2023Article
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- Unique underlying principles shaping copper homeostasis networks.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Review
- The Role of Sulfur in Agronomic Biofortification with Essential Micronutrients.Plants (Basel, Switzerland) · 2022Review
- Interaction between zinc and selenium bio-fortification and toxic metals (loid) accumulation in food crops.Frontiers in plant science · 2022Review
- Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Frontiers in plant science · 2022Article
- Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants.Environmental science and pollution research international · 2021Review
10 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabidopsis thaliana), and functionally characterized this enzyme after heterologous expression in yeast (Saccharomyces cerevisiae). HMA2 is a Zn(2+)-dependent ATPase that is also activated by Cd2+ and, to a lesser extent, by other divalent heavy metals (Pb2+, Ni2+, Cu2+, and Co2+). The enzyme forms an acid-stable phosphorylated intermediate and is inhibited by vanadate. HMA2 interacts with Zn2+ and Cd2+ with high affinity (Zn2+ K(1/2) = 0.11 +/- 0.03 microm and Cd2+ K(1/2) = 0.031 +/- 0.007 microm). However, its activity is dependent on millimolar concentrations of Cys in the assay media. Zn2+ transport determinations indicate that the enzyme drives the outward transport of metals from the cell cytoplasm. Analysis of HMA2 mRNA suggests that the enzyme is present in all plant organs and transcript levels do not change in plants exposed to various metals. Removal of HMA2 full-length transcript results in Zn2+ accumulation in plant tissues. hma2 mutant plants also accumulate Cd2+ when exposed to this metal. These results suggest that HMA2 is responsible for Zn2+ efflux from the cells and therefore is required for maintaining low cytoplasmic Zn2+ levels and normal Zn2+ homeostasis.
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