ArticleJournal of clinical microbiology2004
PCR assay using cerebrospinal fluid for diagnosis of cerebral toxoplasmosis in Brazilian AIDS patients.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 92 citations in OpenAlex.
- Cerebral toxoplasmosis in the twenty-first century.AIDS (London, England) · 2026Observational
- Direct genotype analysis of Toxoplasma gondii in ocular samples from patients with active rtinochoroiditis.BMC infectious diseases · 2025Article
- Diagnostic Challenges and Treatment of Concurrent Toxoplasmosis and Disseminated Cryptococcus in an Immunocompromised Patient.Case reports in infectious diseases · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Seroprevalence and Risk Factors ofFrontiers in cellular and infection microbiology · 2021Article
- Evaluation of a PCR assay for diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell among HIV/AIDS patients.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Evaluation of Serological and Molecular Tests Used for the Identification ofFrontiers in cellular and infection microbiology · 2019Article
- CNS infections in patients with hematological disorders (including allogeneic stem-cell transplantation)-Guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO).Annals of oncology : official journal of the European Society for Medical Oncology · 2016Article
- Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China.Parasites & vectors · 2015Article
- Real-time RT-PCR on SAG1 and BAG1 gene expression during stage conversion in immunosuppressed mice infected with Toxoplasma gondii Tehran strain.The Korean journal of parasitology · 2012Article
- Utility of molecular and serodiagnostic tools in cerebral toxoplasmosis with and without tuberculous meningitis in AIDS patients: A study from South India.Annals of Indian Academy of Neurology · 2010Article
- Importance of nonenteric protozoan infections in immunocompromised people.Clinical microbiology reviews · 2010Review
- Article
- Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients.Cerebrospinal fluid research · 2009Article
- Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strains.Journal of clinical microbiology · 2005Article
- Diagnosis of cerebral toxoplasmosis in AIDS patients in Brazil: importance of molecular and immunological methods using peripheral blood samples.Journal of clinical microbiology · 2005Article
- Evaluation of real-time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid.Journal of veterinary internal medicineArticle
- HIV-Related Cerebral Toxoplasmosis Revisited: Current Concepts and Controversies of an Old Disease.Journal of the International Association of Providers of AIDS CareReview
- Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
- PCR-based Diagnosis ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly active antiretroviral therapy has decreased the incidence of opportunistic infections in the central nervous system in AIDS patients. However, neurological abnormalities still remain important causes of mortality and morbidity in developing countries. In Brazil, cerebral toxoplasmosis is the most common cerebral mass lesion in AIDS patients. For these reasons, early, inexpensive, and sensitive diagnostic tests must be evaluated. The aim of this study was to evaluate PCR, using cerebrospinal fluid (CSF) samples to detect Toxoplasma gondii DNA, and to determine if the association of PCR with immunological assays can contribute to a timely diagnosis. We studied two sample groups. First, we analyzed stored CSF samples from 29 newborns and from 39 adults with AIDS without a definitive diagnosis of toxoplasmosis. The goal of this step was to standardize the methodology with a simple and economical procedure to recover the T. gondii DNA. Next, we prospectively evaluated CSF samples from 12 AIDS patients with a first episode of cerebral toxoplasmosis and 18 AIDS patients with other neurological opportunistic diseases and without previous cerebral toxoplasmosis. In all PCR samples, an indirect immunofluorescent assay and an enzyme-linked immunosorbent assay were performed. Samples from all patients with cerebral toxoplasmosis presented positive PCR results (sensitivity, 100%), and a sample from one of the 18 AIDS patients with other neurological diseases also presented positive PCR results (specificity, 94.4%). These findings suggest the clinical utility of PCR in the diagnosis of cerebral toxoplasmosis in developing countries.
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