ArticleJournal of clinical microbiology2004
Application of molecular fingerprinting for qualitative assessment of small-intestinal bacterial diversity in dogs.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Perspectives and advances in probiotics and the gut microbiome in companion animals.Journal of animal science and technology · 2022Review
- Review
- Characterization of gut microbiomes of household pets in the United States using a direct-to-consumer approach.PloS one · 2020Article
- Managing the Microbial Community of Marine Fish Larvae: A Holistic Perspective for Larviculture.Frontiers in microbiology · 2018Review
- Sanitary impact evaluation of drinking water in storage reservoirs in Moroccan rural area.Saudi journal of biological sciences · 2017Article
- Viable intestinal passage of a canine jejunal commensal strain Lactobacillus acidophilus LAB20 in dogs.Current microbiology · 2014Article
- Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides.Journal of insect science (Online) · 2014Article
- A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples.Journal of microbiological methods · 2013Article
- Ancient T-independence of mucosal IgX/A: gut microbiota unaffected by larval thymectomy in Xenopus laevis.Mucosal immunology · 2013Article
- Characterization of the fungal microbiome (mycobiome) in fecal samples from dogs.Veterinary medicine international · 2013Article
- Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis.BMC veterinary research · 2012Article
- Effect of acid lactic bacteria isolated from faeces of healthy dogs on growth parameters and aflatoxin B1 production by Aspergillus species in vitro.Mycotoxin research · 2011Article
- Companion animals symposium: microbes and gastrointestinal health of dogs and cats.Journal of animal science · 2011Review
- Intestinal microbiota of dogs and cats: a bigger world than we thought.The Veterinary clinics of North America. Small animal practice · 2011Review
- Phylogenetic analysis of 16S rRNA gene sequences reveals distal gut bacterial diversity in wild wolves (Canis lupus).Molecular biology reports · 2010Article
- The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rRNA gene sequencing.BMC microbiology · 2009Article
- Genetic profiling of the oral microbiota associated with severe early-childhood caries.Journal of clinical microbiology · 2007Article
- Endoscopically visualized lesions, histologic findings, and bacterial invasion in the gastrointestinal mucosa of dogs with acute hemorrhagic diarrhea syndrome.Journal of veterinary internal medicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aims of this study were to evaluate the use of molecular fingerprinting for assessment of bacterial diversity in canine duodenal juice and to evaluate the variation in the small intestinal microflora at repeated sampling. Two groups of dogs were used. Duodenal juice was collected from eight dogs euthanized for an unrelated project (group 1). Duodenal juice was also collected endoscopically from six dogs at weekly intervals for a total of 3 weeks (group 2). The variable V6-V8 region of bacterial 16S ribosomal DNA was amplified, and PCR amplicons separated by denaturing gradient gel electrophoresis (DGGE). The reproducibility of DGGE profiles and variations in bacterial diversity between dogs were evaluated by comparing similarity indices (Dice's coefficient; 100% represents complete identity) of DGGE profiles from group 1 dogs. Weekly variations in the flora of the small intestine were evaluated by comparison of DGGE profiles from different time points within the same individuals in group 2. The mean (+/- standard deviation) similarity of DGGE profiles of duodenal juice between the dogs in group 1 was 38.3 +/- 15.7% (range, 12.5 to 76.65%). There was a significantly higher variation in DGGE profiles between different dogs than between duplicates obtained from the same dog (P < 0.0001). DGGE profiles from samples collected at different time points varied within individuals, possibly due to variation over time or slight variation in sampling location. DGGE profiles indicate that dogs have a highly diverse microflora of the small intestine, with marked differences between individual dogs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.