ArticleJournal of clinical microbiology2004
Comparative analysis of Gtf isozyme production and diversity in isolates of Streptococcus mutans with different biofilm growth phenotypes.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
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- The potential use of glycosyl-transferase inhibitors for targeted reduction of S. mutans biofilms in dental materials.Scientific reports · 2023Article
- Inhibition of Biofilm Formation and Virulence Factors of Cariogenic Oral Pathogen Streptococcus mutans by Shikimic Acid.Microbiology spectrum · 2022Article
- Effects of pH on the Properties of Membrane Vesicles Including Glucosyltransferase inMicroorganisms · 2021Article
- Discovery of Potent Inhibitors ofACS medicinal chemistry letters · 2021Article
- Stilbenoids: A Natural Arsenal against Bacterial Pathogens.Antibiotics (Basel, Switzerland) · 2020Review
- Antimicrobial and Antibiofilm Activity againstBioMed research international · 2019Article
- Inhibition ofACS omega · 2018Article
- Whole genome sequence and phenotypic characterization of a CbmMolecular oral microbiology · 2018Article
- Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries.International journal of nanomedicine · 2018Article
- Development and characterization of p1025-loaded bioadhesive liquid-crystalline system for the prevention ofInternational journal of nanomedicine · 2018Article
- Exploring the Genomic Diversity and Cariogenic Differences of Streptococcus mutans Strains Through Pan-Genome and Comparative Genome Analysis.Current microbiology · 2017Article
- Structure-Based Discovery of Small Molecule Inhibitors of Cariogenic Virulence.Scientific reports · 2017Article
- Mechanistic, genomic and proteomic study on the effects of BisGMA-derived biodegradation product on cariogenic bacteria.Dental materials : official publication of the Academy of Dental Materials · 2017Article
- Hydroxychalcone inhibitors of Streptococcus mutans glucosyl transferases and biofilms as potential anticaries agents.Bioorganic & medicinal chemistry letters · 2016Article
- Molecule Targeting Glucosyltransferase Inhibits Streptococcus mutans Biofilm Formation and Virulence.Antimicrobial agents and chemotherapy · 2016Article
- Polyphenol-Rich Extract from Propolis Reduces the Expression and Activity of Streptococcus mutans Glucosyltransferases at Subinhibitory Concentrations.BioMed research international · 2016Article
- Oral lactic acid bacteria related to the occurrence and/or progression of dental caries in Japanese preschool children.Bioscience of microbiota, food and health · 2015Article
- Antibiofilm Activity of Chilean Propolis on Streptococcus mutans Is Influenced by the Year of Collection.BioMed research international · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Streptococcus mutans is the main pathogenic agent of dental caries. Glucosyltransferases (Gtfs) produced by these bacteria are important virulence factors because they catalyze the extracellular synthesis of glucans that are necessary for bacterial accumulation in the dental biofilm. The diversity of GtfB and GtfC isozymes was analyzed in 44 genotypes of S. mutans that showed a range of abilities to form biofilms in vitro. Several approaches were used to characterize these isozymes, including restriction fragment length polymorphism analysis of the gtfB and gtfC genes, zymographic analysis of the identified GtfB and GtfC genotypes, and quantitation of isozyme production in immunoblot experiments with specific monoclonal antibodies. A high diversity of gtf genes, patterns of enzymatic activity, and isozyme production was identified among the isolates tested. GtfC and, to a lesser extent, GtfB were produced in significantly higher amounts by strains that had high biofilm-forming ability than by strains with low biofilm-forming ability. Biofilm formation was independent of the GtfB and GtfC genotype. Atypical strains that showed an apparent single Gtf isozyme of intermediate size between GtfB and GtfC were also identified. The results indicate that various expression levels of GtfB and GtfC isozymes are associated with the ability of distinct S. mutans genotypes to grow as biofilms, strengthening the results of previous genetic and biochemical studies performed with laboratory strains. These studies also emphasize the need to identify factors that control gtf gene expression.
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