ArticleJournal of clinical microbiology2004
Instability of the restriction fragment length polymorphism pattern of open reading frame 5 of porcine reproductive and respiratory syndrome virus during sequential pig-to-pig passages.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Geographic Distribution and ORF5 Diversity of PRRSV-2 Variants in Midwestern U.S. Diagnostic Submissions, 2023-2025.Pathogens (Basel, Switzerland) · 2026Article
- Genetic diversity and pathogenicity of novel recombinant strains of PRRSV lineages 1.5, lineages 1.8 and lineages 8 in South China.Frontiers in cellular and infection microbiology · 2026Article
- In Vitro Characterization of Vaccine Strain-like Porcine Reproductive and Respiratory Syndrome Virus Strains Isolated from Weaned Pigs Exhibiting Respiratory Symptoms.Pathogens (Basel, Switzerland) · 2025Article
- Molecular characterization of porcine reproductive and respiratory syndrome virus in Henan and Shanxi, China, during 2023-2024.Archives of virology · 2025Article
- Review
- PRRSV-2 variant classification: a dynamic nomenclature for enhanced monitoring and surveillance.mSphere · 2025Article
- Refining Lineage Classification and Updated RFLP Patterns of PRRSV-2 Revealed Viral Spatiotemporal Distribution Characteristics in China in 1991-2023.Transboundary and emerging diseases · 2025Article
- Epidemiology and Pathogenicity Analysis Based on Partial Recombinant PRRSV Strains in China.Transboundary and emerging diseases · 2025Article
- Unveiling the Anticancer Potential of a New Ciprofloxacin-Chalcone Hybrid as an Inhibitor of Topoisomerases I & II and Apoptotic Inducer.Molecules (Basel, Switzerland) · 2024Article
- Rapid PRRSV-2 ORF5-based lineage classification using Nextclade.Frontiers in veterinary science · 2024Article
- Molecular characterization of porcine reproductive and respiratory syndrome virus identified in 2021 from Nepal.Frontiers in veterinary science · 2024Article
- Refining PRRSV-2 genetic classification based on global ORF5 sequences and investigation of their geographic distributions and temporal changes.Microbiology spectrum · 2023Article
- Article
- Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study.Viruses · 2023Article
- Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus.Frontiers in microbiology · 2023Review
- Phylogenetic analysis of porcine reproductive and respiratory syndrome virus in Vietnam, 2021.Virus genes · 2022Article
- Molecular Evolution of Porcine Reproductive and Respiratory Syndrome Virus Field Strains from Two Swine Production Systems in the Midwestern United States from 2001 to 2020.Microbiology spectrum · 2022Article
- Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017-2022.Frontiers in microbiology · 2022Article
- Epidemiological and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in South China Between 2017 and 2021.Frontiers in veterinary science · 2022Article
- First Detection of NADC34-like PRRSV as a Main Epidemic Strain on a Large Farm in China.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction fragment length polymorphism (RFLP) analysis is one of the tools commonly used to study the molecular epidemiology of porcine reproductive and respiratory syndrome viruses (PRRSVs). As PRRSVs are genetically variable, the stability of the RFLP pattern of a PRRSV during in vivo replication was evaluated by carrying out 13 sequential pig-to-pig passages (P1 to P13) of PRRSV ATCC VR-2332 in three independent pig lines for a total of 727 days. During P1 the pigs were inoculated with a homogeneous inoculum (CC-01) prepared through a series of plaque purifications, and during P2 to P13 the pigs were inoculated with a tissue filtrate from the corresponding pig in the previous passage. Fifteen viral plaque clones were directly isolated from CC-01 and the day 7 serum of each pig of each passage, open reading frame 5 of the clones was sequenced, and the clones were compared to CC-01 to assess the mutation rates and RFLP patterns (obtained by digestion with MluI, HincII, and SacII) over time. Among the 495 viral clones recovered during the passages, 398 clones, including CC-01, had pattern 2-5-2 (MluI-HincII-SacII); however, the remaining 97 viral clones showed different patterns (2-6-2 [P2], 1-5-2 [P3], 2-5-4 [P7], and 2-1-2 [P10]). Importantly, the MluI site that was reported to be present in only one of the PRRS modified live virus vaccine strains (Ingelvac) and its parental strain (ATCC VR-2332) can disappear during in vivo replication. Furthermore, sequence homology between CC-01 and clones with pattern 2-5-2 or clones with other patterns differed by 0.05 to 1.58% and 0.5 to 1.45%, respectively, suggesting that RFLP analysis cannot accurately predict genetic relatedness between PRRSVs. Collectively, precaution should be taken when the molecular epidemiology of PRRSVs is evaluated by RFLP analysis.
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