ArticleThe EMBO journal2004
Reorganisation of an RNA polymerase-promoter DNA complex for DNA melting.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Analysis and comparison of the bacterial σ54 regulon: Evidence of phylogenetic trends in gene regulation.PloS one · 2025Article
- Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.Nucleic acids research · 2014Article
- The σ70 region 1.2 regulates promoter escape by unwinding DNA downstream of the transcription start site.Nucleic acids research · 2013Article
- The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.Microbiology and molecular biology reviews : MMBR · 2012Review
- An integrated chemical cross-linking and mass spectrometry approach to study protein complex architecture and function.Molecular & cellular proteomics : MCP · 2012Article
- A prehydrolysis state of an AAA+ ATPase supports transcription activation of an enhancer-dependent RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The role of the conserved phenylalanine in the sigma54-interacting GAFTGA motif of bacterial enhancer binding proteins.Nucleic acids research · 2009Article
- Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein.Molecular microbiology · 2009Article
- Comparative analysis of activator-Esigma54 complexes formed with nucleotide-metal fluoride analogues.Nucleic acids research · 2009Article
- Coupling sigma factor conformation to RNA polymerase reorganisation for DNA melting.Journal of molecular biology · 2009Article
- Organization of an activator-bound RNA polymerase holoenzyme.Molecular cell · 2008Article
- Regulated communication between the upstream face of RNA polymerase and the beta' subunit jaw domain.The EMBO journal · 2004Article
- Nucleotide-dependent interactions between a fork junction-RNA polymerase complex and an AAA+ transcriptional activator protein.Nucleic acids research · 2004Article
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Authors and funding
4 authors.
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Abstract
Sigma factors, the key regulatory components of the bacterial RNA polymerase (RNAP), direct promoter DNA binding and DNA melting. The sigma(54)-RNAP forms promoter complexes in which DNA melting is only triggered by an activator and ATP hydrolysis-driven reorganisation of an initial sigma(54)-RNAP-promoter complex. We report that an initial bacterial RNAP-DNA complex can be reorganised by an activator to form an intermediate transcription initiation complex where full DNA melting has not yet occurred. Using sigma(54) as a chemical nuclease we now show that the reorganisation of the initial sigma(54)-RNAP-promoter complex occurs upon interaction with the activator at the transition point of ATP hydrolysis. We demonstrate that this reorganisation event is an early step in the transcription initiation pathway that occurs independently of RNAP parts normally associated with stable DNA melting and open complex formation. Using photoreactive DNA probes, we provide evidence that within this reorganised sigma(54)-RNAP-promoter complex, DNA contacts across the 'to be melted' sequences are made by the sigma(54) subunit. Strikingly, the activator protein, but not core RNAP subunits, is close to these DNA sequences.
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