ArticleThe EMBO journal2004
Regulated communication between the upstream face of RNA polymerase and the beta' subunit jaw domain.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.Nucleic acids research · 2017Article
- The bacterial enhancer-dependent RNA polymerase.The Biochemical journal · 2016Review
- The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism.Journal of molecular biology · 2016Article
- Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis.Journal of molecular biology · 2015Article
- TRANSCRIPTION. Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies.Science (New York, N.Y.) · 2015Article
- A non-bacterial transcription factor inhibits bacterial transcription by a multipronged mechanism.RNA biology · 2013Article
- A dual switch controls bacterial enhancer-dependent transcription.Nucleic acids research · 2012Article
- Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2.Molecular cell · 2012Article
- T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Coupling sigma factor conformation to RNA polymerase reorganisation for DNA melting.Journal of molecular biology · 2009Article
- Organization of an activator-bound RNA polymerase holoenzyme.Molecular cell · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
We used bacteriophage T7-encoded transcription inhibitor gene protein 2 (gp2) as a probe to study the contribution of the Escherichia coli RNA polymerase (RNAP) beta' subunit jaw domain--the site of gp2 binding--to activator and ATP hydrolysis-dependent open complex formation by the sigma(54)-RNAP. We show that, unlike sigma(70)-dependent transcription, activated transcription by sigma(54)-RNAP is resistant to gp2. In contrast, activator and ATP hydrolysis-independent transcription by sigma(54)-RNAP is highly sensitive to gp2. We provide evidence that an activator- and ATP hydrolysis-dependent conformational change involving the beta' jaw domain and promoter DNA is the basis for gp2-resistant transcription by sigma(54)-RNAP. Our results establish that accessory factors bound to the upstream face of the RNAP, communicate with the beta' jaw domain, and that such communication is subjected to regulation.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.