ArticleEukaryotic cell2004
RNA polymerase II localizes in Tetrahymena thermophila meiotic micronuclei when micronuclear transcription associated with genome rearrangement occurs.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena.Science China. Life sciences · 2026Article
- A specialized TFIIB is required for transcription of transposon-targeting noncoding RNAs.Nucleic acids research · 2025Article
- The PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Paramecium.Nucleic acids research · 2025Article
- RebL1 is required for macronuclear structure stability and gametogenesis inMarine life science & technology · 2024Article
- A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination.eLife · 2024Article
- The Conjusome-A Transient Organelle Linking Genome Rearrangements in the Parental and Developing Macronuclei.Microorganisms · 2023Article
- A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic BacteriumMicroorganisms · 2023Article
- The transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development.Nucleic acids research · 2022Article
- A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies.Nucleic acids research · 2021Article
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Nucleus-specific linker histones Hho1 and Mlh1 form distinct protein interactions during growth, starvation and development in Tetrahymena thermophila.Scientific reports · 2020Article
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- RNAi-dependentGenes & development · 2019Article
- The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila.Epigenetics & chromatin · 2018Article
- RNA-mediated transgenerational inheritance in ciliates and plants.Chromosoma · 2018Review
- Whats, hows and whys of programmed DNA elimination inOpen biology · 2017Review
- A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.Nucleic acids research · 2017Article
- Article
- Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin.Nucleic acids research · 2016Article
- Small-RNA-Mediated Genome-wide trans-Recognition Network in Tetrahymena DNA Elimination.Molecular cell · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The germ line micronucleus in Tetrahymena thermophila is transcriptionally silent in vegetatively growing cells. However, micronuclear transcription has been observed in the early ("crescent") stages of the sexual process, conjugation. This transcription is proposed to play a central role in identifying sites for subsequent genome rearrangements that accompany development of the somatic macronucleus from the micronucleus. RPB3 (cnjC), a gene encoding a protein homologous to the third largest subunit of RNA polymerase II (RNAP II), was previously reported to be expressed specifically during conjugation, suggesting a role in micronucleus-specific transcription. Rpb3p localized in the micronucleus only during the meiotic prophase, when micronuclear transcription occurs, and its intranuclear distribution is strikingly similar to that for previously described sites of micronuclear RNA synthesis. By contrast, Rpc5p, the homologous subunit shared by RNAPs I and III, was not detectable in the micronucleus at any stage of the life cycle. However, Rpb3p is not specific to the transcribing micronucleus. Like Rpc5p, it also localizes to macronuclei in all stages of the life cycle. Rpb3p is encoded by a unique, essential gene in Tetrahymena. Thus, RNAP II is associated with both somatic transcription and crescent transcription and probably has an important role in genome rearrangement.
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Registered trials
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