ArticleEukaryotic cell2004
Pmt-mediated O mannosylation stabilizes an essential component of the secretory apparatus, Sec20p, in Candida albicans.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Extension ofmBio · 2022Article
- Monitoring Protein Dynamics in ProteinMolecules (Basel, Switzerland) · 2018Article
- Mapping the O-Mannose Glycoproteome in Saccharomyces cerevisiae.Molecular & cellular proteomics : MCP · 2016Article
- Article
- Aspergillus nidulans Pmts form heterodimers in all pairwise combinations.FEBS open bio · 2014Article
- Identification of O-mannosylated virulence factors in Ustilago maydis.PLoS pathogens · 2012Article
- Dissecting the biological role of mucin-type O-glycosylation using RNA interference in Drosophila cell culture.The Journal of biological chemistry · 2010Article
- Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans.Eukaryotic cell · 2009Article
- Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta.Applied and environmental microbiology · 2008Article
- O-Mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) is crucial for cell wall integrity and conidium morphology, especially at an elevated temperature.Eukaryotic cell · 2007Article
- Virulence of the fungal pathogen Candida albicans requires the five isoforms of protein mannosyltransferases.Infection and immunity · 2005Article
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Authors and funding
3 authors.
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Abstract
Sec20p is an essential endoplasmic reticulum (ER) membrane protein in yeasts, functioning as a tSNARE component in retrograde vesicle traffic. We show that Sec20p in the human fungal pathogen Candida albicans is extensively O mannosylated by protein mannosyltransferases (Pmt proteins). Surprisingly, Sec20p occurs at wild-type levels in a pmt6 mutant but at very low levels in pmt1 and pmt4 mutants and also after replacement of specific Ser/Thr residues in the lumenal domain of Sec20p. Pulse-chase experiments revealed rapid degradation of unmodified Sec20p (38.6 kDa) following its biosynthesis, while the stable O-glycosylated form (50 kDa) was not formed in a pmt1 mutant. These results suggest a novel function of O mannosylation in eukaryotes, in that modification by specific Pmt proteins will prevent degradation of ER-resident membrane proteins via ER-associated degradation or a proteasome-independent pathway.
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