ArticleEukaryotic cell2004
Introns and splicing elements of five diverse fungi.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.
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Who cites it
136 citing papers in PubMed, 312 citations in OpenAlex.
- Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing.eLife · 2026Article
- Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes.Current research in microbial sciences · 2026Review
- Propagation of [D1,2]-type spliceosomal twin introns (stwintrons) inMicrobiology spectrum · 2025Article
- Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling.Cell genomics · 2025Article
- Cryptococcal nutrient acquisition and pathogenesis: dining on the host.Microbiology and molecular biology reviews : MMBR · 2025Review
- From computational models of the splicing code to regulatory mechanisms and therapeutic implications.Nature reviews. Genetics · 2025Review
- LUC7 proteins define two major classes of 5' splice sites in animals and plants.Nature communications · 2025Article
- Biochemical and inhibitor analysis of recombinant cellobiohydrolases from Phanerochaete chrysosporium.Biotechnology for biofuels and bioproducts · 2024Article
- Genes divided according to the relative position of the longest intron show increased representation in different KEGG pathways.BMC genomics · 2024Article
- Article
- A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast.PLoS genetics · 2024Article
- An interpretable model of pre-mRNA splicing for animal and plant genes.Science advances · 2024Article
- Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 inInternational journal of molecular sciences · 2024Article
- Genome Comparisons betweenJournal of fungi (Basel, Switzerland) · 2024Article
- Near chromosome-level genome assembly of the microsporidium Hamiltosporidium tvaerminnensis.G3 (Bethesda, Md.) · 2023Article
- The Biological Action and Structural Characterization of Eryngitin 3 and 4, Ribotoxin-like Proteins fromInternational journal of molecular sciences · 2023Article
- A genetic tool to express long fungal biosynthetic genes.Fungal biology and biotechnology · 2023Article
- Genome-wide identification of the opsin protein inFrontiers in microbiology · 2023Article
- Quantitative and qualitative plant-pathogen interactions call upon similar pathogenicity genes with a spectrum of effects.Frontiers in plant science · 2023Article
- A broadly conserved fungal alcohol oxidase (AOX) facilitates fungal invasion of plants.Molecular plant pathology · 2023Article
76 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Genomic sequences and expressed sequence tag data for a diverse group of fungi (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus nidulans, Neurospora crassa, and Cryptococcus neoformans) provided the opportunity to accurately characterize conserved intronic elements. An examination of large intron data sets revealed that fungal introns in general are short, that 98% or more of them belong to the canonical splice site (ss) class (5'GU...AG3'), and that they have polypyrimidine tracts predominantly in the region between the 5' ss and the branch point. Information content is high in the 5' ss, branch site, and 3' ss regions of the introns but low in the exon regions adjacent to the introns in the fungi examined. The two yeasts have broader intron length ranges and correspondingly higher intron information content than the other fungi. Generally, as intron length increases in the fungi, so does intron information content. Homologs of U2AF spliceosomal proteins were found in all species except for S. cerevisiae, suggesting a nonconventional role for U2AF in the absence of canonical polypyrimidine tracts in the majority of introns. Our observations imply that splicing in fungi may be different from that in vertebrates and may require additional proteins that interact with polypyrimidine tracts upstream of the branch point. Theoretical protein homologs for Nam8p and TIA-1, two proteins that require U-rich regions upstream of the branch point to function, were found. There appear to be sufficient differences between S. cerevisiae and S. pombe introns and the introns of two filamentous members of the Ascomycota and one member of the Basidiomycota to warrant the development of new model organisms for studying the splicing mechanisms of fungi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.