ArticleMolecular biology of the cell2004
Regulation of epidermal growth factor receptor degradation by heterotrimeric Galphas protein.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
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- EFR3A: a new raft domain organizing protein?Cellular & molecular biology letters · 2023Article
- Endosomal cargo recycling mediated by Gpa1 and phosphatidylinositol 3-kinase is inhibited by glucose starvation.Molecular biology of the cell · 2022Article
- Genotypes of Pain and Analgesia in a Randomized Trial of Irritable Bowel Syndrome.Frontiers in psychiatry · 2022Article
- SNX-PXA-RGS-PXC Subfamily of SNXs in the Regulation of Receptor-Mediated Signaling and Membrane Trafficking.International journal of molecular sciences · 2021Review
- GαProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Gαs regulates Glucagon-Like Peptide 1 Receptor-mediated cyclic AMP generation at Rab5 endosomal compartment.Molecular metabolism · 2017Article
- ARF6 Is an Actionable Node that Orchestrates Oncogenic GNAQ Signaling in Uveal Melanoma.Cancer cell · 2016Article
- Mutations in SNX14 cause a distinctive autosomal-recessive cerebellar ataxia and intellectual disability syndrome.American journal of human genetics · 2014Article
- Structural basis for different phosphoinositide specificities of the PX domains of sorting nexins regulating G-protein signaling.The Journal of biological chemistry · 2014Article
- Gαs regulates the post-endocytic sorting of G protein-coupled receptors.Nature communications · 2014Article
- Gαs protein expression is an independent predictor of recurrence in prostate cancer.Journal of immunology research · 2014Article
- A novel regulatory function of sweet taste-sensing receptor in adipogenic differentiation of 3T3-L1 cells.PloS one · 2013Article
- Gαs promotes EEA1 endosome maturation and shuts down proliferative signaling through interaction with GIV (Girdin).Molecular biology of the cell · 2012Article
- Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- G protein trafficking.Sub-cellular biochemistry · 2012Review
- Non-canonical signaling and localizations of heterotrimeric G proteins.Cellular signalling · 2012Review
- Tyrosine phosphorylation of the Gα-interacting protein GIV promotes activation of phosphoinositide 3-kinase during cell migration.Science signaling · 2011Article
- Phox homology band 4.1/ezrin/radixin/moesin-like proteins function as molecular scaffolds that interact with cargo receptors and Ras GTPases.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Girding for migratory cues: roles of the Akt substrate Girdin in cancer progression and angiogenesis.Cancer science · 2010Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Heterotrimeric G proteins have been implicated in the regulation of membrane trafficking, but the mechanisms involved are not well understood. Here, we report that overexpression of the stimulatory G protein subunit (Galphas) promotes ligand-dependent degradation of epidermal growth factor (EGF) receptors and Texas Red EGF, and knock-down of Galphas expression by RNA interference (RNAi) delays receptor degradation. We also show that Galphas and its GTPase activating protein (GAP), RGS-PX1, interact with hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs), a critical component of the endosomal sorting machinery. Galphas coimmunoprecipitates with Hrs and binds Hrs in pull-down assays. By immunofluorescence, exogenously expressed Galphas colocalizes with myc-Hrs and GFP-RGS-PX1 on early endosomes, and expression of either Hrs or RGS-PX1 increases the localization of Galphas on endosomes. Furthermore, knock-down of both Hrs and Galphas by double RNAi causes greater inhibition of EGF receptor degradation than knock-down of either protein alone, suggesting that Galphas and Hrs have cooperative effects on regulating EGF receptor degradation. These observations define a novel regulatory role for Galphas in EGF receptor degradation and provide mechanistic insights into the function of Galphas in endocytic sorting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.