ArticleMolecular biology of the cell2004
Direct visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 63 citations in OpenAlex.
- Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes.PloS one · 2024Article
- Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment.Cell reports · 2022Article
- Directly Measuring Forces within Reconstituted Active Microtubule Bundles.Journal of visualized experiments : JoVE · 2022Article
- Mechanical Mechanisms of Chromosome Segregation.Cells · 2021Review
- Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.The EMBO journal · 2020Article
- Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B.Molecular biology of the cell · 2019Article
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
- Tension sensors reveal how the kinetochore shares its load.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Review
- Mitosis.Cold Spring Harbor perspectives in biology · 2016Review
- Using Photobleaching to Measure Spindle Microtubule Dynamics in Primary Cultures of Dividing Drosophila Meiotic Spermatocytes.Journal of biomolecular techniques : JBT · 2015Article
- From equator to pole: splitting chromosomes in mitosis and meiosis.Genes & development · 2015Review
- Kinetochore-driven outgrowth of microtubules is a central contributor to kinetochore fiber maturation in crane-fly spermatocytes.Molecular biology of the cell · 2014Article
- Regulation of chromosome speeds in mitosis.Cellular and molecular bioengineering · 2013Article
- Review
- Review series: The functions and consequences of force at kinetochores.The Journal of cell biology · 2013Review
- Pac-man motility of kinetochores unleashed by laser microsurgery.Molecular biology of the cell · 2012Article
- Deformations within moving kinetochores reveal different sites of active and passive force generation.Science (New York, N.Y.) · 2012Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule flux in spindles of insect spermatocytes, long-used models for studies on chromosome behavior during meiosis, was revealed after iontophoretic microinjection of rhodamine-conjugated (rh)-tubulin and fluorescent speckle microscopy. In time-lapse movies of crane-fly spermtocytes, fluorescent speckles generated when rh-tubulin incorporated at microtubule plus ends moved poleward through each half-spindle and then were lost from microtubule minus ends at the spindle poles. The average poleward velocity of approximately 0.7 microm/min for speckles within kinetochore microtubules at metaphase increased during anaphase to approximately 0.9 microm/min. Segregating half-bivalents had an average poleward velocity of approximately 0.5 microm/min, about half that of speckles within shortening kinetochore fibers. When injected during anaphase, rhtubulin was incorporated at kinetochores, and kinetochore fiber fluorescence spread poleward as anaphase progressed. The results show that tubulin subunits are added to the plus end of kinetochore microtubules and are removed from their minus ends at the poles, all while attached chromosomes move poleward during anaphase A. The results cannot be explained by a Pac-man model, in which 1) kinetochore-based, minus end-directed motors generate poleward forces for anaphase A and 2) kinetochore microtubules shorten at their plus ends. Rather, in these cells, kinetochore fiber shortening during anaphase A occurs exclusively at the minus ends of kinetochore microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.