ArticleBMC genomics2004
Evaluation of sense-strand mRNA amplification by comparative quantitative PCR.
Article in BMC genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
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- RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts.BMC research notes · 2009Article
- Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling.BMC genomics · 2009Article
- Terminal continuation (TC) RNA amplification without second strand synthesis.Journal of neuroscience methods · 2009Article
- Using differential gene expression to study Entamoeba histolytica pathogenesis.Trends in parasitology · 2009Review
- Terminal Continuation (TC) RNA amplification enables expression profiling using minute RNA input obtained from mouse brain.International journal of molecular sciences · 2008Article
- Comparison of RNA amplification techniques meeting the demands for the expression profiling of clinical cancer samples.Virchows Archiv : an international journal of pathology · 2007Article
- Single molecule transcription profiling with AFM.Nanotechnology · 2007Article
- Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT).Nucleic acids research · 2007Article
- Characterization of ovine hepatic gene expression profiles in response to Escherichia coli lipopolysaccharide using a bovine cDNA microarray.BMC veterinary research · 2006Article
- Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies.Molecular cancer · 2006Article
- Comparative evaluation of linear and exponential amplification techniques for expression profiling at the single-cell level.Genome biology · 2006Article
- Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling.Nucleic acids research · 2006Review
- Cryptococcus neoformans gene expression during murine macrophage infection.Eukaryotic cell · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA amplification is required for incorporating laser-capture microdissection techniques into microarray assays. However, standard oligonucleotide microarrays contain sense-strand probes, so traditional T7 amplification schemes producing anti-sense RNA are not appropriate for hybridization when combined with conventional reverse transcription labeling methods. We wished to assess the accuracy of a new sense-strand RNA amplification method by comparing ratios between two samples using quantitative real-time PCR (qPCR), mimicking a two-color microarray assay.
resultsWe performed our validation using qPCR. Three samples of rat brain RNA and three samples of rat liver RNA were amplified using several kits (Ambion messageAmp, NuGen Ovation, and several versions of Genisphere SenseAmp). Results were assessed by comparing the liver/brain ratio for 192 mRNAs before and after amplification. In general, all kits produced strong correlations with unamplified RNAs. The SenseAmp kit produced the highest correlation, and was also able to amplify a partially degraded sample accurately.
conclusionWe have validated an optimized sense-strand RNA amplification method for use in comparative studies such as two-color microarrays.
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