ReviewGlycoconjugate journal2004
Expression of human glycosyltransferase genes in yeast as a tool for enzymatic synthesis of sugar chain.
Review in Glycoconjugate journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 13 citations in OpenAlex.
- In VitroJournal of microbiology and biotechnology · 2021Article
- Implications on glycobiological aspects of tumor hypoxia in breast ductal carcinoma in situ.Medical molecular morphology · 2013Article
- Evaluation of glycophenotype in breast cancer by quantum dot-lectin histochemistry.International journal of nanomedicine · 2013Article
- Autoantibodies to recombinant human CTL2 in autoimmune hearing loss.The Laryngoscope · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We planned the production of human glycosyltransferases in yeast for the enzymatic synthesis of various sugar chains. More than 160 genes encoding various glycosyltransferases were prepared as N-terminal transmembrane region truncated forms by PCR and were inserted into the entry vector of Invitrogen Ltd's Gateway system. About fifty glycosyltransferases were chosen for the synthesis of human type oligosaccharides, and expressed as two different forms in yeast. One is a soluble form, which is secreted into the culture medium by methylotrophic yeast, and the other is an immobilized form, which is displayed at the budding yeast cell wall as a fusion protein with Pir protein. To date, in both systems, some sialyltranferases and fucosyltransferases have been produced as active forms, indicating the potential usefulness of these systems for the enzymatic synthesis of various types of human sugar chains attached to proteins and lipids.
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Registered trials
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