ArticleJournal of biomedicine & biotechnology2004
Osteogenic Cells Derived From Embryonic Stem Cells Produced Bone Nodules in Three-Dimensional Scaffolds.
Article in Journal of biomedicine & biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Application of selected scaffolds for bone tissue engineering: a systematic review.Oral and maxillofacial surgery · 2017Pooled it
- Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.Journal of bone and mineral metabolism · 2017Article
- Simplified three-dimensional culture system for long-term expansion of embryonic stem cells.World journal of stem cells · 2015Article
- Simvastatin promotes osteogenic differentiation of mouse embryonic stem cells via canonical Wnt/β-catenin signaling.Molecules and cells · 2011Article
- Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation.Head & face medicine · 2011Article
- Enhancing osteogenic differentiation of mouse embryonic stem cells by nanofibers.Tissue engineering. Part A · 2009Article
- The influence of three-dimensional nanofibrous scaffolds on the osteogenic differentiation of embryonic stem cells.Biomaterials · 2009Article
- Induction of osteogenic markers in differentially treated cultures of embryonic stem cells.Head & face medicine · 2008Article
- Historic and current strategies in bone tissue engineering: do we have a hope in Hench?Journal of materials science. Materials in medicine · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An approach for 3D bone tissue generation from embryonic stem (ES) cells was investigated. The ES cells were induced to differentiate into osteogenic precursors, capable of proliferating and subsequently differentiating into bone-forming cells. The differentiated cells and the seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron microscopy, and RT-PCR analysis. The results demonstrated that ES-derived bone-forming cells attached to and colonized the biocompatible and biodegradable scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not an ES cell-specific marker, oct-4. These findings suggest that ES cell can be used for in vitro tissue engineering and cultivation of graftable skeletal structures.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.