ArticleApplied and environmental microbiology2004
Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Insight into Shiga toxin genes encoded by Escherichia coli O157 from whole genome sequencing.PeerJ · 2015Article
- Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature.Journal of clinical microbiology · 2012Article
- Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool.Diagnostic microbiology and infectious disease · 2012Article
- Differential virulence of clinical and bovine-biased enterohemorrhagic Escherichia coli O157:H7 genotypes in piglet and Dutch belted rabbit models.Infection and immunity · 2012Article
- Multilocus sequence typing and virulence factors analysis of Escherichia coli O157 strains in China.Journal of microbiology (Seoul, Korea) · 2010Article
- Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2010Article
- Identification and characterization of Shiga toxin type 2 variants in Escherichia coli isolates from animals, food, and humans.Applied and environmental microbiology · 2008Article
- Epidemiological survey on Escherichia coli O157 in Chongqing and Three-Gorge Reservoir Areas of China.Veterinary research communications · 2008Article
- Tracing shigatoxigenic Escherichia coli O103, O145, and O174 infections from farm residents to cattle.Journal of clinical microbiology · 2007Article
- Analysis of the clonal relationship of shiga toxin-producing Escherichia coli serogroup O165:H25 isolated from cattle.Applied and environmental microbiology · 2006Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxins Stx1 and Stx2 play a prominent role in the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections. Several variants of the stx(2) gene, encoding Stx2, have been described. In this study, we developed a PCR-restriction fragment length polymorphism system for typing stx(2) genes of STEC strains. The typing system discriminates eight described variants and allows the identification of new stx(2) variants and STEC isolates carrying multiple stx(2) genes. A phylogenetic tree, based on the nucleotide sequences of the toxin-encoding genes, demonstrates that stx(2) sequences with the same PvuII HaeIII HincII AccI type generally cluster together.
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