ArticlePlant physiology2004
Biochemical characterization of the tobacco 42-kD protein kinase activated by osmotic stress.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 68 citations in OpenAlex.
- Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice.Cell discovery · 2022Article
- Growth Promotion or Osmotic Stress Response: How SNF1-Related Protein Kinase 2 (SnRK2) Kinases Are Activated and Manage Intracellular Signaling in Plants.Plants (Basel, Switzerland) · 2021Review
- Role of Raf-like kinases in SnRK2 activation and osmotic stress response in plants.Nature communications · 2020Review
- Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- From Survival to Productivity Mode: Cytokinins Allow Avoiding the Avoidance Strategy Under Stress Conditions.Frontiers in plant science · 2020Article
- SnRK2 Protein Kinases and mRNA Decapping Machinery Control Root Development and Response to Salt.Plant physiology · 2020Article
- Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.).BMC genetics · 2017Article
- Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.).BMC genetics · 2017Article
- Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase.BMC plant biology · 2016Article
- Differential Activation of the Wheat SnRK2 Family by Abiotic Stresses.Frontiers in plant science · 2016Article
- Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 ofBioMed research international · 2016Article
- Abscisic Acid and Abiotic Stress Tolerance in Crop Plants.Frontiers in plant science · 2016Review
- Differential expression ofMolecular biology research communications · 2014Article
- Cloning and characterization of SnRK2 subfamily II genes from Nicotiana tabacum.Molecular biology reports · 2014Article
- The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress.The Plant journal : for cell and molecular biology · 2012Article
- SNF1-related protein kinases type 2 are involved in plant responses to cadmium stress.Plant physiology · 2012Article
- Reactive oxygen species are involved in gibberellin/abscisic acid signaling in barley aleurone cells.Plant physiology · 2012Article
- Abscisic acid signaling: thermal stability shift assays as tool to analyze hormone perception and signal transduction.PloS one · 2012Article
- Article
- SnRK2 protein kinases--key regulators of plant response to abiotic stresses.Omics : a journal of integrative biology · 2011Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In tobacco (Nicotiana tabacum), hyperosmotic stress induces rapid activation of a 42-kD protein kinase, referred to as Nicotiana tabacum osmotic stress-activated protein kinase (NtOSAK). cDNA encoding the kinase was cloned and, based on the predicted amino acid sequence, the enzyme was assigned to the SNF1-related protein kinase type 2 (SnRK2) family. The identity of the enzyme was confirmed by immunoprecipitation of the active kinase from tobacco cells subjected to osmotic stress using antibodies raised against a peptide corresponding to the C-terminal sequence of the kinase predicted from the cloned cDNA. A detailed biochemical characterization of NtOSAK purified from stressed tobacco cells was performed. Our results show that NtOSAK is a calcium-independent Ser/Thr protein kinase. The sequence of putative phosphorylation sites recognized by NtOSAK, predicted by the computer program PREDIKIN, resembled the substrate consensus sequence defined for animal and yeast (Saccharomyces cerevisiae) AMPK/SNF1 kinases. Our experimental data confirmed these results, as various targets for AMPK/SNF1 kinases were also efficiently phosphorylated by NtOSAK. A range of protein kinase inhibitors was tested as potential modulators of NtOSAK, but only staurosporine, a rather nonspecific protein kinase inhibitor, was found to abolish the enzyme activity. In phosphorylation reactions, NtOSAK exhibited a preference for Mg(2+) over Mn(2+) ions and an inability to use GTP instead of ATP as a phosphate donor. The enzyme activity was not modulated by 5'-AMP. To our knowledge, these results represent the first detailed biochemical characterization of a kinase of the SnRK2 family.
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