ArticleJournal of bacteriology2004
Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Transcriptome data are insufficient to control false discoveries in regulatory network inference.Cell systems · 2024Article
- Interactions between the AraC/XylS-like transcriptional activator InvF of Salmonella Typhimurium, the RNA polymerase alpha subunit and the chaperone SicA.Scientific reports · 2024Article
- Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1.Biotechnology for biofuels and bioproducts · 2023Article
- Redefining fundamental concepts of transcription initiation in bacteria.Nature reviews. Genetics · 2020Review
- Rapid method for chromatin immunoprecipitation (ChIP) assay in a dimorphic fungus, Candida albicans.Journal of microbiology (Seoul, Korea) · 2020Article
- Transcription of Bacterial Chromatin.Journal of molecular biology · 2019Review
- Transcription factor YcjW controls the emergency HNature communications · 2019Article
- Unusually Situated Binding Sites for Bacterial Transcription Factors Can Have Hidden Functionality.PloS one · 2016Article
- Integration of AI-2 Based Cell-Cell Signaling with Metabolic Cues in Escherichia coli.PloS one · 2016Article
- D101 is critical for the function of AttJ, a repressor of quorum quenching system in Agrobacterium tumefaciens.Journal of microbiology (Seoul, Korea) · 2015Article
- Transcriptional analysis of the MrpJ network: modulation of diverse virulence-associated genes and direct regulation of mrp fimbrial and flhDC flagellar operons in Proteus mirabilis.Infection and immunity · 2015Article
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- The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY.Applied and environmental microbiology · 2014Article
- Transcriptional regulator LsrB of Sinorhizobium meliloti positively regulates the expression of genes involved in lipopolysaccharide biosynthesis.Applied and environmental microbiology · 2014Article
- The effect of cell growth phase on the regulatory cross-talk between flagellar and Spi1 virulence gene expression.PLoS pathogens · 2014Article
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- Pro-inflammatory cytokines can act as intracellular modulators of commensal bacterial virulence.Open biology · 2013Article
- "Non-canonical protein-DNA interactions identified by ChIP are not artifacts": response.BMC genomics · 2013Article
- Article
- Integrated stress response of Escherichia coli to methylglyoxal: transcriptional readthrough from the nemRA operon enhances protection through increased expression of glyoxalase I.Molecular microbiology · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli MelR protein is a transcription activator that is essential for melibiose-dependent expression of the melAB genes. We have used chromatin immunoprecipitation to study the binding of MelR and RNA polymerase to the melAB promoter in vivo. Our results show that MelR is associated with promoter DNA, both in the absence and presence of the inducer melibiose. In contrast, RNA polymerase is recruited to the melAB promoter only in the presence of inducer. The MelR DK261 positive control mutant binds to the melAB promoter but cannot recruit RNA polymerase. Further analysis of immunoprecipitated DNA, by using an Affymetrix GeneChip array, showed that the melAB promoter is the major, if not the sole, target in E. coli for MelR. This was confirmed by a transcriptomics experiment to analyze RNA in cells either with or without melR.
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