ArticleJournal of bacteriology2004
Thermophilic, reversible gamma-resorcylate decarboxylase from Rhizobium sp. strain MTP-10005: purification, molecular characterization, and expression.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 57 citations in OpenAlex.
- Functional heterologous expression of the reversibleEngineering microbiology · 2025Article
- Enzymatic Conversion of COChemical reviews · 2023Review
- Metal Ion Promiscuity and Structure of 2,3-Dihydroxybenzoic Acid Decarboxylase of Aspergillus oryzae.Chembiochem : a European journal of chemical biology · 2021Article
- Novel 3,6-Dihydroxypicolinic Acid Decarboxylase-Mediated Picolinic Acid Catabolism inJournal of bacteriology · 2019Article
- Mechanism and Structure of γ-Resorcylate Decarboxylase.Biochemistry · 2018Article
- Pressurized COGreen chemistry : an international journal and green chemistry resource : GC · 2018Article
- C-H Carboxylation of Aromatic Compounds through COChemSusChem · 2017Review
- Biogenetic Relationships of Bioactive Sponge Merotriterpenoids.Marine drugs · 2017Review
- Functional Characterization of a Novel Member of the Amidohydrolase 2 Protein Family, 2-Hydroxy-1-Naphthoic Acid Nonoxidative Decarboxylase from Burkholderia sp. Strain BC1.Journal of bacteriology · 2016Article
- γ-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.Applied and environmental microbiology · 2015Article
- Regioselective Enzymatic β-Carboxylation ofAdvanced synthesis & catalysis · 2015Article
- Biomimetic Synthesis of Cbz-(S)-Dolaphenine.Tetrahedron letters · 2012Article
- Regioselective enzymatic carboxylation of phenols and hydroxystyrene derivatives.Organic letters · 2012Article
- Characterization of a novel type of oxidative decarboxylase involved in the biosynthesis of the styryl moiety of chondrochloren from an acylated tyrosine.The Journal of biological chemistry · 2010Article
- Uncovering the protocatechuate 2,3-cleavage pathway genes.Journal of bacteriology · 2009Article
- Crystallization and preliminary X-ray diffraction studies of maleylacetate reductase from Rhizobium sp. strain MTP-10005.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Biochemical and genetic analysis of the gamma-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster.Journal of bacteriology · 2007Article
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We found the occurrence of thermophilic reversible gamma-resorcylate decarboxylase (gamma-RDC) in the cell extract of a bacterium isolated from natural water, Rhizobium sp. strain MTP-10005, and purified the enzyme to homogeneity. The molecular mass of the enzyme was determined to be about 151 kDa by gel filtration, and that of the subunit was 37.5 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; in other words, the enzyme was a homotetramer. The enzyme was induced specifically by the addition of gamma-resorcylate to the medium. The enzyme required no coenzyme and did not act on 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3,5-dihydroxybenzoate, 2-hydroxybenzoate, or 3-hydroxybenzoate. It was relatively thermostable to heat treatment, and its half-life at 50 degrees C was estimated to be 122 min; furthermore, it catalyzed the reverse carboxylation of resorcinol. The values of k(cat)/K(m) (mMu(-1) . s(-1)) for gamma-resorcylate and resorcinol at 30 degrees C and pH 7 were 13.4 and 0.098, respectively. The enzyme contains 327 amino acid residues, and sequence identities were found with those of hypothetical protein AGR C 4595p from Agrobacterium tumefaciens strain C58 (96% identity), 5-carboxyvanillate decarboxylase from Sphingomonas paucimobilis (32%), and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylases from Bacillus cereus ATCC 10987 (26%), Rattus norvegicus (26%), and Homo sapiens (25%). The genes (graA [1,230 bp], graB [888 bp], and graC [1,056 bp]) that are homologous to those in the resorcinol pathway also exist upstream and downstream of the gamma-RDC gene. Judging from these results, the resorcinol pathway also exists in Rhizobium sp. strain MTP-10005, and gamma-RDC probably catalyzes a reaction just before the hydroxylase in it does.
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