ArticleGenome biology2004
A genome annotation-driven approach to cloning the human ORFeome.
Article in Genome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Article
- Review
- KDEL Receptors: Pathophysiological Functions, Therapeutic Options, and Biotechnological Opportunities.Biomedicines · 2022Review
- Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Predicting Drug-Target Interactions Based on the Ensemble Models of Multiple Feature Pairs.International journal of molecular sciences · 2021Article
- BCL2L13: physiological and pathological meanings.Cellular and molecular life sciences : CMLS · 2021Review
- Computational Modeling of C-Terminal Tails to Predict the Calcium-Dependent Secretion of Endoplasmic Reticulum Resident Proteins.Frontiers in chemistry · 2021Article
- CRISPR/Cas9 mediated knock-out of VPREB1 gene induces a cytotoxic effect in myeloma cells.PloS one · 2021Article
- KDELR2 Promotes Glioblastoma Tumorigenesis Targeted by HIF1a via mTOR Signaling Pathway.Cellular and molecular neurobiology · 2019Article
- KDEL Receptors Are Differentially Regulated to Maintain the ER Proteome under Calcium Deficiency.Cell reports · 2018Article
- Identification and characterization of a Golgi retention signal in feline coronavirus accessory protein 7b.The Journal of general virology · 2017Article
- Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling.BioMed research international · 2015Article
- Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells.Methods (San Diego, Calif.) · 2012Article
- Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts.Genome biology · 2010Article
- Large-scale screening for novel low-affinity extracellular protein interactions.Genome research · 2008Article
- A biomedically enriched collection of 7000 human ORF clones.PloS one · 2008Article
- Finishing the finished human chromosome 22 sequence.Genome biology · 2008Article
- The UCSC Genome Browser Database: 2008 update.Nucleic acids research · 2008Article
- Multiplexed expression and screening for recombinant protein production in mammalian cells.BMC biotechnology · 2006Article
- Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression.BMC biotechnology · 2004Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.