ArticleGenome biology2004
Development of a method for screening short-lived proteins using green fluorescent protein.
Article in Genome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- An "OFF-ON-OFF" fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems.Chemical science · 2022Article
- Proteomics: Concepts and applications in human medicine.World journal of biological chemistry · 2021Review
- Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells.International journal of molecular sciences · 2021Article
- The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression.Nucleic acids research · 2019Article
- Corrected and Republished from: The COP9 Signalosome Interacts with and Regulates Interferon Regulatory Factor 5 Protein Stability.Molecular and cellular biology · 2018Article
- Article
- Psoriatic arthritis under a proteomic spotlight: application of novel technologies to advance diagnosis and management.Current rheumatology reports · 2015Review
- The COP9 signalosome interacts with and regulates interferon regulatory factor 5 protein stability.Molecular and cellular biology · 2013Article
- Article
- Papillorenal syndrome-causing missense mutations in PAX2/Pax2 result in hypomorphic alleles in mouse and human.PLoS genetics · 2010Article
- Evaluation of GFP tag as a screening reporter in directed evolution of a hyperthermophilic beta-glucosidase.Molecular biotechnology · 2009Article
- Recent advances in proteomics and cancer biomarker discovery.Clinical medicine. Oncology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library were screened to recover those in which the fluorescence signal diminished rapidly when protein synthesis was inhibited. Thirty clones that met the screening criteria were characterized individually. Twenty-three (73%) proved to have a half-life of 4 hours or less.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.