ArticlePlant cell reports2005
Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 168 citations in OpenAlex.
- Breeding of glyphosate-tolerant genetically modified rice BriA15-38.GM crops & food · 2026Article
- BcMFSR Is Required for Efficient Uptake of Exogenous dsRNA and Spray-Induced Gene Silencing in Botrytis cinerea.Molecular plant pathology · 2026Article
- Intrinsically disordered regions restrain genomic targeting of RNA and histone demethylases in mammals and plants.Nature genetics · 2026Article
- Genome-wide regulatory and interaction landscape underlying the functional divergence of rice AGAMOUS-like transcription factors.Journal of experimental botany · 2026Article
- OsvWA36 transcriptionally regulates cell wall remodeling to control grain length in rice.BMC plant biology · 2026Article
- From Microbial Consortia to Ecosystem Resilience: The Integrative Roles of Holobionts in Stress Biology.Biology · 2025Article
- CRISPR-Cas13a as a next-generation tool for rapid and precise plant RNA virus diagnostics.Plant methods · 2025Review
- Review of the technology used for structural characterization of the GMO genome using NGS data.Genomics & informatics · 2024Article
- Alternative splicing of a potato disease resistance gene maintains homeostasis between growth and immunity.The Plant cell · 2024Article
- Stress-induced nuclear translocation of ONAC023 improves drought and heat tolerance through multiple processes in rice.Nature communications · 2024Article
- XA21-mediated resistance toPeerJ · 2024Article
- Identifying transgene insertions inPeerJ · 2024Article
- Differential editing efficiencies in cereal crops: a comparative analysis of tRNA and ribozyme multiplexed guide delivery.Frontiers in plant science · 2024Article
- Analysis of the Candidate Genes and Underlying Molecular Mechanism of P198, an RNAi-Related Dwarf and Sterile Line.International journal of molecular sciences · 2023Article
- SHORT ROOT and INDETERMINATE DOMAIN family members govern PIN-FORMED expression to regulate minor vein differentiation in rice.The Plant cell · 2023Article
- An EfficientPlants (Basel, Switzerland) · 2023Article
- Proof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes.Scientific reports · 2023Article
- Insights into enhancingFrontiers in plant science · 2023Article
- An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice.Frontiers in plant science · 2023Article
- High-frequency synthetic apomixis in hybrid rice.Nature communications · 2022Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In transgenic plants, transgene copy number can greatly influence the expression level and genetic stability of the target gene, making estimation of transgene copy number an important area of genetically modified (GM) crop research. Transgene copy numbers are currently estimated by Southern analysis, which is laborious and time-consuming, requires relatively large amounts of plant materials and may involve hazardous radioisotopes. We report here the development of a sensitive, high-throughput real-time (RT)-PCR technique for estimating transgene copy number in GM rice. This system uses TaqMan quantitative RT-PCR and comparison to a novel rice endogenous reference gene coding for sucrose phosphate synthase (SPS) to determine the copy numbers of the exogenous beta-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes in transgenic rice. The copy numbers of the GUS and HPT in primary rice transformants (T0) were calculated by comparing quantitative PCR results of the GUS and HPT genes with those of the internal standard, SPS. With optimized PCR conditions, we achieved significantly accurate estimates of one, two, three and four transgene copies in the T0 transformants. Furthermore, our copy number estimations of both the GUS reporter gene and the HPT selective marker gene showed that rearrangements of the T-DNA occurred more frequently than is generally believed in transgenic rice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.