ArticleNucleic acids research2004
The Saccharomyces cerevisiae TRT2 tRNAThr gene upstream of STE6 is a barrier to repression in MATalpha cells and exerts a potential tRNA position effect in MATa cells.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- tRNAs as a Driving Force of Genome Evolution in Yeast.Frontiers in microbiology · 2021Review
- Overlapping but Distinct Sequences Play Roles in the Insulator and Promoter Activities of theGenetics · 2020Article
- tRNA Genes Affect Chromosome Structure and Function via Local Effects.Molecular and cellular biology · 2019Article
- Remarkably Long-Tract Gene Conversion Induced by Fragile Site Instability in Saccharomyces cerevisiae.Genetics · 2016Article
- Yeast high mobility group protein HMO1 stabilizes chromatin and is evicted during repair of DNA double strand breaks.Nucleic acids research · 2015Article
- Compromised RNA polymerase III complex assembly leads to local alterations of intergenic RNA polymerase II transcription in Saccharomyces cerevisiae.BMC biology · 2014Article
- A novel tRNA variable number tandem repeat at human chromosome 1q23.3 is implicated as a boundary element based on conservation of a CTCF motif in mouse.Nucleic acids research · 2014Article
- Intergenic transcriptional interference is blocked by RNA polymerase III transcription factor TFIIIB in Saccharomyces cerevisiae.Genetics · 2014Article
- Autoregulation of an RNA polymerase II promoter by the RNA polymerase III transcription factor III C (TF(III)C) complex.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A cis-acting tRNA gene imposes the cell cycle progression requirement for establishing silencing at the HMR locus in yeast.Genetics · 2011Article
- tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica.Eukaryotic cell · 2010Article
- Article
- A role for non-coding Tsix transcription in partitioning chromatin domains within the mouse X-inactivation centre.Epigenetics & chromatin · 2009Article
- Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation.Nucleic acids research · 2009Article
- Limiting the extent of the RDN1 heterochromatin domain by a silencing barrier and Sir2 protein levels in Saccharomyces cerevisiae.Molecular and cellular biology · 2009Article
- TFIIIC binding sites function as both heterochromatin barriers and chromatin insulators in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1.PloS one · 2007Article
- Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Modulation of yeast genome expression in response to defective RNA polymerase III-dependent transcription.Molecular and cellular biology · 2005Article
- TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs.Genes & development · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA molecules, certain RNA polymerase III genes also function as genomic landmarks. Transfer RNA genes are known to exhibit extra-transcriptional activities such as directing Ty element integration, pausing of replication forks, overriding nucleosome positioning sequences, repressing neighboring genes (tRNA position effect), and acting as a barrier to the spread of repressive chromatin. This study was designed to identify other tRNA loci that may act as barriers to chromatin-mediated repression, and focused on TRT2, a tRNA(Thr) adjacent to the STE6 alpha2 operator. We show that TRT2 acts as a barrier to repression, protecting the upstream CBT1 gene from the influence of the STE6 alpha2 operator in MATalpha cells. Interestingly, deletion of TRT2 results in an increase in CBT1 mRNA levels in MATa cells, indicating a potential tRNA position effect. The transcription of TRT2 itself is unaffected by the presence of the alpha2 operator, suggesting a hierarchy that favors assembly of the RNA polymerase III complex versus assembly of adjacent alpha2 operator-mediated repressed chromatin structures. This proposed hierarchy could explain how tRNA genes function as barriers to the propagation of repressive chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.