ArticleNucleic acids research2004
A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- Why AGG is associated with high transgene output: passenger effects and their implications for transgene design.NAR genomics and bioinformatics · 2025Article
- Azithromycin represses evolution of ceftazidime/avibactam resistance by translational repression ofJournal of bacteriology · 2025Article
- Increasing recombinant protein production in E. coli via FACS-based selection of N-terminal coding DNA libraries.The FEBS journal · 2025Article
- Genes for highly abundant proteins in Escherichia coli avoid 5' codons that promote ribosomal initiation.PLoS computational biology · 2023Article
- Initiation at AUGUG and GUGUG sequences can lead to translation of overlapping reading frames in E. coli.Nucleic acids research · 2023Article
- Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression.Microbial cell factories · 2018Article
- Chemical complexity of protein determines optimalJournal, genetic engineering & biotechnology · 2017Article
- Heterologous production of curcuminoids.Microbiology and molecular biology reviews : MMBR · 2015Review
- A novel methodology for enhanced and consistent heterologous expression of unmodified human cytochrome P450 1B1 (CYP1B1).PloS one · 2014Article
- Design and optimization of short DNA sequences that can be used as 5' fusion partners for high-level expression of heterologous genes in Escherichia coli.Applied and environmental microbiology · 2013Article
- Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins.Microbial cell factories · 2013Review
- Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.Journal of biomolecular NMR · 2013Article
- Strategies of bacterial over expression of membrane transporters relevant in human health: the successful case of the three members of OCTN subfamily.Molecular biotechnology · 2013Review
- Recycling of peptidyl-tRNAs by peptidyl-tRNA hydrolase counteracts azithromycin-mediated effects on Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2013Article
- Capreomycin susceptibility is increased by TlyA-directed 2'-O-methylation on both ribosomal subunits.Molecular microbiology · 2012Article
- An unstructured 5'-coding region of the prfA mRNA is required for efficient translation.Nucleic acids research · 2012Article
- Disruptive mRNA folding increases translational efficiency of catechol-O-methyltransferase variant.Nucleic acids research · 2011Article
- Reduced stability of mRNA secondary structure near the translation-initiation site in dsDNA viruses.BMC evolutionary biology · 2011Article
- Article
- Influence of the second amino acid on recombinant protein expression.Protein expression and purification · 2010Article
Corrections and comments
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.