ArticleNucleic acids research2004
Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed.
- Childhood environment influences epigenetic age and methylation concordance of a CpG clock locus in British-Bangladeshi migrants.Epigenetics · 2023Article
- Methods to improve the accuracy of next-generation sequencing.Frontiers in bioengineering and biotechnology · 2023Review
- Expanding the DNA-encoded library toolbox: identifying small molecules targeting RNA.Nucleic acids research · 2022Article
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation.Epigenetics · 2021Article
- The Opportunities and Challenges of Molecular Tagging Next-Generation Sequencing in Liquid Biopsy.Molecular diagnosis & therapy · 2021Article
- A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol.Nucleic acids research · 2021Article
- Comparison of Target Enrichment Platforms for Circulating Tumor DNA Detection.Scientific reports · 2020Article
- Systematic comparison of somatic variant calling performance among different sequencing depth and mutation frequency.Scientific reports · 2020Article
- eVIDENCE: a practical variant filtering for low-frequency variants detection in cell-free DNA.Scientific reports · 2019Article
- Graphical Workflow System for Modification Calling by Machine Learning of Reverse Transcription Signatures.Frontiers in genetics · 2019Article
- The Pipeline Repertoire for Ig-Seq Analysis.Frontiers in immunology · 2019Review
- Cellular barcoding: lineage tracing, screening and beyond.Nature methods · 2018Review
- Two are better than one: HPoxBS - hairpin oxidative bisulfite sequencing.Nucleic acids research · 2018Article
- Unique Molecular Identifiers reveal a novel sequencing artefact with implications for RNA-Seq based gene expression analysis.Scientific reports · 2018Article
- Partial bisulfite conversion for unique template sequencing.Nucleic acids research · 2018Article
- Epigenetic memory via concordant DNA methylation is inversely correlated to developmental potential of mammalian cells.PLoS genetics · 2017Article
- How to deduplicate PCR.Nature methods · 2017Article
- Limitations and challenges of genetic barcode quantification.Scientific reports · 2017Article
- Whole-organism lineage tracing by combinatorial and cumulative genome editing.Science (New York, N.Y.) · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular 'batch-stamps' that effectively label each genomic template with a sample ID and analysis date. This highly sensitive method identifies redundant and contaminant sequences and serves as a reliable method for positive identification of desired sequences; we can therefore capture accurately the genomic template diversity in the sample analyzed. Although our application described here involves the use of hairpin-bisulfite PCR for amplification of double-stranded DNA, the method can readily be adapted to single-strand PCR. Useful applications will include analyses of limited template DNA for biomedical, ancient DNA and forensic purposes.
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Registered trials
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