ArticleMolecular and cellular biology1992
The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 51 citations in OpenAlex.
- Multiplex assay detection of immunoglobulin G antibodies that recognize Giardia intestinalis and Cryptosporidium parvum antigens.Clinical and vaccine immunology : CVI · 2010Article
- Biology of Giardia lamblia.Clinical microbiology reviews · 2001Review
- Immune response to Giardia duodenalis.Clinical microbiology reviews · 2000Review
- Kinetoplastid glucose transporters.The Biochemical journal · 1997Review
- Serological analysis of antigenic heterogeneity of Giardia lamblia variant surface proteins.Infection and immunity · 1996Article
- Allele-specific expression of a variant-specific surface protein (VSP) of Giardia lamblia.Nucleic acids research · 1994Article
- Size heterogeneity among antigenically related Giardia lamblia variant-specific surface proteins is due to differences in tandem repeat copy number.Infection and immunity · 1994Article
- cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a candidate proprotein convertase expressed in endocrine and nonendocrine cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Variant-specific surface proteins of Giardia lamblia are zinc-binding proteins.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Spontaneous chromosome rearrangements in the protozoan Giardia lamblia: estimation of mutation rates.Nucleic acids research · 1992Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Giardia lamblia trophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier studies, we cloned a portion of the gene for a 170-kDa surface antigen (CRP170) and demonstrated by DNA sequencing that it was cysteine rich (12%) and contained 2.6 copies of a tandemly repeated 195-bp pair sequence. The clone hybridized to multiple bands on a Southern blot of G. lamblia DNA in a pattern that was variable among the cloned lines but did not correlate with expression of CRP170. We have now cloned a nearly full length cDNA as well as genomic clones for CRP170 from the WBA6 cloned isolate. In addition, we have isolated a cDNA clone from the WB1269 line (expressing CRP72), an antigenic variant which was derived from WBA6. Sequence analysis of the CRP170 and CRP72 genes revealed marked C-terminal amino acid homology, suggesting a conserved functional role such as membrane anchoring. The CRP170 repeat oligonucleotide hybridized to a stairstep of bands approximately 6 kb in size on HindIII-digested WBA6 DNA representing the expressed copy(ies) of CRP170. In contrast, there was no hybridization to a fragment of similar size in WB1269, suggesting that WB1269 trophozoites have lost the expressed copy of the CRP170 gene.
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