ArticleMolecular and cellular biology2004
RNA polymerase II carboxy-terminal domain phosphorylation is required for cotranscriptional pre-mRNA splicing and 3'-end formation.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 126 citations in OpenAlex.
- Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.Nature communications · 2025Article
- Coupling of alternative splicing and alternative polyadenylation.Acta biochimica et biophysica Sinica · 2024Review
- Pre-mRNA splicing and its cotranscriptional connections.Trends in genetics : TIG · 2023Review
- Position-dependent effects of RNA-binding proteins in the context of co-transcriptional splicing.NPJ systems biology and applications · 2023Article
- RNA Metabolism in T Lymphocytes.Immune network · 2022Review
- Transcriptional CDK Inhibitors as Potential Treatment Option for Testicular Germ Cell Tumors.Cancers · 2022Article
- Oncogenic dysregulation of pre-mRNA processing by protein kinases: challenges and therapeutic opportunities.The FEBS journal · 2021Review
- Tyrosine kinase inhibitors induce alternative spliced BCR-ABLCancer science · 2020Article
- Regulation of the Ras-Related Signaling Pathway by Small Molecules Containing an Indole Core Scaffold: A Potential Antitumor Therapy.Frontiers in pharmacology · 2020Review
- Distinct mechanistic responses to replication fork stalling induced by either nucleotide or protein deprivation.Cell cycle (Georgetown, Tex.) · 2018Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- Exploring the Impact of Cleavage and Polyadenylation Factors on Pre-mRNA Splicing Across Eukaryotes.G3 (Bethesda, Md.) · 2017Article
- Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus.The Journal of cell biology · 2016Article
- Relationship between nucleosome positioning and progesterone-induced alternative splicing in breast cancer cells.RNA (New York, N.Y.) · 2015Article
- Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.Molecular and cellular biology · 2014Article
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- Characterization of molecular and cellular functions of the cyclin-dependent kinase CDK9 using a novel specific inhibitor.British journal of pharmacology · 2014Article
- Transcriptome-wide modulation of splicing by the exon junction complex.Genome biology · 2014Article
- CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation.RNA biology · 2014Article
- Reflections on the history of pre-mRNA processing and highlights of current knowledge: a unified picture.RNA (New York, N.Y.) · 2013Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the role of RNA polymerase II (pol II) carboxy-terminal domain (CTD) phosphorylation in pre-mRNA processing coupled and uncoupled from transcription in Xenopus oocytes. Inhibition of CTD phosphorylation by the kinase inhibitors 5,6-dichloro-1beta-D-ribofuranosyl-benzimidazole and H8 blocked transcription-coupled splicing and poly(A) site cleavage. These experiments suggest that pol II CTD phosphorylation is required for efficient pre-mRNA splicing and 3'-end formation in vivo. In contrast, processing of injected pre-mRNA was unaffected by either kinase inhibitors or alpha-amanitin-induced depletion of pol II. pol II therefore does not appear to participate directly in posttranscriptional processing, at least in frog oocytes. Together these experiments show that the influence of the phosphorylated CTD on pre-mRNA splicing and 3'-end processing is mediated by transcriptional coupling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.