ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
ATP-induced non-neuronal cell permeabilization in the rat inner retina.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Adenosine triphosphate-induced cell death in heart failure: Is there a link?World journal of cardiology · 2025Review
- Molecular and metabolic landscape of adenosine triphosphate-induced cell death in cardiovascular disease.World journal of cardiology · 2024Review
- Ionotropic purinergic receptor 7 (P2X7) channel structure and pharmacology provides insight regarding non-nucleotide agonism.Channels (Austin, Tex.) · 2024Review
- Toxigenic Clostridium perfringens Isolated from At-Risk Paediatric Inflammatory Bowel Disease Patients.Journal of Crohn's & colitis · 2024Article
- Extracellular histone proteins activate P2XR7 channel current.The Journal of general physiology · 2023Article
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Article
- Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.eLife · 2018Article
- Involvement of P2X7 receptor in neuronal degeneration triggered by traumatic injury.Scientific reports · 2016Article
- P2XPurinergic signalling · 2016Article
- Article
- Saffron reduces ATP-induced retinal cytotoxicity by targeting P2X7 receptors.Purinergic signalling · 2016Article
- Activated Müller Cells Involved in ATP-Induced Upregulation of P2XBioMed research international · 2016Article
- Role of P2X7 receptors in the development of diabetic retinopathy.World journal of diabetes · 2014Review
- Molecular pathways of pannexin1-mediated neurotoxicity.Frontiers in physiology · 2014Review
- ATP induces the death of developing avian retinal neurons in culture via activation of P2X7 and glutamate receptors.Purinergic signalling · 2013Article
- Purinergic trophic signalling in glial cells: functional effects and modulation of cell proliferation, differentiation, and death.Purinergic signalling · 2012Review
- Aspects of astrocyte energy metabolism, amino acid neurotransmitter homoeostasis and metabolic compartmentation.ASN neuro · 2012Review
- The P2X(7) receptor in retinal ganglion cells: A neuronal model of pressure-induced damage and protection by a shifting purinergic balance.Purinergic signalling · 2009Article
- The P2X(7) receptor in retinal ganglion cells: A neuronal model of pressure-induced damage and protection by a shifting purinergic balance.Purinergic signalling · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The P2X7 subtype holds a special position among P2X receptors because of its ability to act both as a classical, ligand-gated ion channel, and as a permeabilization pore that can induce cell death under prolonged activation by ATP. We have shown previously that, in rat retina, P2X7 receptors are located in the inner nuclear layer and ganglion cell layer (GCL). The present study was aimed at finding whether retinal P2X7 receptors can act as a mediator of cell permeabilization and, if so, at identifying the cellular target(s) of this effect. As an indicator of cell permeabilization, we used the fluorescent dye YO-PRO-1 (molecular weight, 375 Da), which enters cells only through large pores like those opened by prolonged or sustained stimulation of P2X(7) receptors and binds to DNA, providing a stable labeling of the activated cells. Different agonists for P2 receptors were tested for their ability to cause cell permeabilization in flat-mounted rat retinas. Among them, only high concentrations of ATP (500 microM) and BzATP (2',3'-O-(4-benzoyl-benzoyl)-ATP triethylammonium) (100 microM) were able to induce accumulation of YO-PRO-1 in the GCL and in the nerve fiber layer, suggesting that different cell types were responding to P2X7 stimulation. This effect was blocked by the P2 antagonists suramin and PPADS (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid) and by the P2X7-selective inhibitor Brilliant Blue G. To identify the retinal cell types affected by ATP-induced permeabilization, we used in vivo labeling techniques. Our data clearly reveal that prolonged stimulation of P2X7 receptors elicits permeabilization exclusively in microglial cells but not in neurons of the inner retina.
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