ReviewGene therapy2004
DNA electrotransfer: its principles and an updated review of its therapeutic applications.
Review in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed, 284 citations in OpenAlex.
- Advances in gene transfer technologies: comparing viral and non-viral vectors for therapeutic applications.3 Biotech · 2026Review
- Unleashing CAR-T potential in solid tumors: overcoming intrinsic and extrinsic hurdles to improve therapy.Cancer immunology, immunotherapy : CII · 2026Review
- Effect of Bleomycin Hydrolase Expression in Tumor Tissue on the Therapeutic Effectiveness of Electrochemotherapy.Cancers · 2025Article
- Optimization of in vivo delivery methods and their applications in seminiferous tubules of mice.BMC biotechnology · 2025Article
- Electroporation in Cancer Therapy: A Simplified Model Derived from the Hodgkin-Huxley Model.Bioelectricity · 2024Article
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- GM1 asymmetry in the membrane stabilizes pores.Biophysical journal · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Modeling the gene delivery process of the needle array-based tissue nanotransfection.Nano research · 2022Article
- Gene Therapy of Chronic Limb-Threatening Ischemia: Vascular Medical Perspectives.Journal of clinical medicine · 2022Review
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- A review of the tortuous path of nonviral gene delivery and recent progress.International journal of biological macromolecules · 2021Review
- Drug Delivery via the Suprachoroidal Space for the Treatment of Retinal Diseases.Pharmaceutics · 2021Review
- A DNA-based vaccine protects against Crimean-Congo haemorrhagic fever virus disease in a Cynomolgus macaque model.Nature microbiology · 2021Article
- Genetic Modification of Mesenchymal Stem Cells for Neurological Disease Therapy: What Effects Does it Have on Phenotype/Cell Behavior, Determining Their Effectiveness?Molecular diagnosis & therapy · 2020Review
- Electroporation as the Immunotherapy Strategy for Cancer in Veterinary Medicine: State of the Art in Latin America.Vaccines · 2020Review
- Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation.Microsystems & nanoengineering · 2020Article
- 3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.Lab on a chip · 2019Article
- A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle.International journal of molecular sciences · 2019Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of electric pulses to transfect all types of cells is well known and regularly used in vitro for bacteria and eukaryotic cells transformation. Electric pulses can also be delivered in vivo either transcutaneously or with electrodes in direct contact with the tissues. After injection of naked DNA in a tissue, appropriate local electric pulses can result in a very high expression of the transferred genes. This manuscript describes the evolution in the concepts and the various optimization steps that have led to the use of combinations of pulses that fit with the known roles of the electric pulses in DNA electrotransfer, namely cell electropermeabilization and DNA electrophoresis. A summary of the main applications published until now is also reported, restricted to the in vivo preclinical trials using therapeutic genes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.