ArticleBiophysical journal2004
Estimating the sensitivity of mechanosensitive ion channels to membrane strain and tension.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Parallelized Mechanical Stimulation of Neuronal Calcium Through Cell-Internal Nanomagnetic Forces Provokes Lasting Shifts in the Network Activity State.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Research progress on the regulatory role of cell membrane surface tension in cell behavior.Heliyon · 2024Review
- Investigation of nanotopography on SOCE mediated cell migration via live-cell : Imaging on opaque implant surface.Journal of nanobiotechnology · 2023Article
- Alterations in the microenvironment and the effects produced of TRPV5 in osteoporosis.Journal of translational medicine · 2023Review
- The plasma membrane as a mechanochemical transducer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- An influenza-derived membrane tension-modulating peptide regulates cell movement and morphology via actin remodeling.Communications biology · 2019Article
- Interrogating the Electromechanical Regulation of Cellular Volume at the Single-Cell Level.Biophysical journal · 2018Article
- Cell Mechanotransduction With Piconewton Forces Applied by Optical Tweezers.Frontiers in cellular neuroscience · 2018Article
- Bone and skeletal muscle: Key players in mechanotransduction and potential overlapping mechanisms.Bone · 2015Review
- Efficient Exploration of Membrane-Associated Phenomena at Atomic Resolution.The Journal of membrane biology · 2015Review
- Stochastic nanoroughness modulates neuron-astrocyte interactions and function via mechanosensing cation channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Lamin B1 overexpression increases nuclear rigidity in autosomal dominant leukodystrophy fibroblasts.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Nanobiomechanics of living cells: a review.Interface focus · 2014Review
- Local membrane deformation and micro-injury lead to qualitatively different responses in osteoblasts.F1000Research · 2014Article
- Use the force: membrane tension as an organizer of cell shape and motility.Trends in cell biology · 2013Review
- Human AQP1 is a constitutively open channel that closes by a membrane-tension-mediated mechanism.Biophysical journal · 2013Article
- Mechanical characterization of adult stem cells from bone marrow and perivascular niches.Journal of biomechanics · 2012Article
- Intracellular Ca(2+) accumulation is strain-dependent and correlates with apoptosis in aortic valve fibroblasts.Journal of biomechanics · 2012Article
- Odontoblasts in developing, mature and ageing rat teeth have multiple phenotypes that variably express all nine voltage-gated sodium channels.Archives of oral biology · 2011Article
- Adipose-derived stem cells in functional bone tissue engineering: lessons from bone mechanobiology.Tissue engineering. Part B, Reviews · 2011Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone adapts to its environment by a process in which osteoblasts and osteocytes sense applied mechanical strain. One possible pathway for the detection of strain involves mechanosensitive channels and we sought to determine their sensitivity to membrane strain and tension. We used a combination of experimental and computational modeling techniques to gain new insights into cell mechanics and the regulation of mechanosensitive channels. Using patch-clamp electrophysiology combined with video microscopy, we recorded simultaneously the evolution of membrane extensions into the micropipette, applied pressure, and membrane currents. Nonselective mechanosensitive cation channels with a conductance of 15 pS were observed. Bleb aspiration into the micropipette was simulated using finite element models incorporating the cytoplasm, the actin cortex, the plasma membrane, cellular stiffening in response to strain, and adhesion between the membrane and the micropipette. Using this model, we examine the relative importance of the different cellular components in resisting suction into the pipette and estimate the membrane strains and tensions needed to open mechanosensitive channels. Radial membrane strains of 800% and tensions of 5 10(-4) N.m(-1) were needed to open 50% of mechanosensitive channels. We discuss the relevance of these results in the understanding of cellular reactions to mechanical strain and bone physiology.
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